2011 Fiscal Year Final Research Report
Development of a diagnostic method for malignant hyperthermia indexed by cell death provoked by stimulation of ryanodine 1 receptors in monocytes
| Project/Area Number |
22791453
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Saitama Medical University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Keywords | 麻酔学 / 悪性高熱症 / リアノジン受容体 / ブピバカイン / カフェイン / ミトコンドリア / 細胞障害 / ネクローシス |
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| Research Abstract |
To develop a new diagnostic method for malignant hyperthermia, utility of necrotic cytotoxicity assay provoked by agonists for ryanodine-1 receptors(RyR1) in monocytes was evaluated. Two RyR1 agonists, bupivacaine as well as caffeine, elevated intracellular Ca^<2+> concentration. However, bupivacaine but not caffeine provoked cell death. Differential effects of these RyR1 agonists on mitochondrial membrane potential mimicked those on necrotic cell death. These indicated that intracellular Ca^<2+> mobilization was not the direct cause of cell death provoked by RyR1 agonists. Further evaluation concerning the relationship between mitochondrial damage and necrosis provoked by RyR1 agonists should be required.
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