Development of novel intravesical agents for the treatment of refractory superficial bladder cancer

2011 Fiscal Year Final Research Report

• Project/Area Number: 22791473
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Urology
• Research Institution: Okayama University
• Principal Investigator: WATANABE Masami 岡山大学, 岡山大学病院, 准教授 (70444677)
• Project Period (FY): 2010 – 2011
• Keywords: 膀胱癌 / 遺伝子治療 / 抗癌免疫 / 分化

Research Abstract

Bladder cancer is one of the most common urogenital malignancies. The intravesical instillation of anti-cancer agent is an attractive strategy to treat a superficial lesion or floating/disseminated cancer cells after transurethral operation. An adenovirus carrying REIC/Dkk-3, a tumor suppressor gene,(Ad-REIC) exhibits cancer-specific apoptotic effects in various types of cancer cells. In this study, we demonstrated that the intravesical instillation of Ad-REIC could be an attractive therapeutic method for the treatment of human bladder cancer.

Research Products

• [Journal Article] Partial sensitization of human bladder cancer cells to a gene-therapeutic adenovirus carrying REIC/Dkk-3 by downregulation of BRPK/PINK1 (2012)
  • Author(s): Jin Y, Mur ata H, Sakaguchi M, Kataoka K, Watanabe M, Nasu Y, Kumon H, Huh NH
  • Journal Title: Oncol Rep Volume: 27(3) Pages: 695-9
  • DOI: doi:10.3892/or.2011.1543.Epub2011Nov10
  • Peer Reviewed
• [Journal Article] Preclinical safety and efficacy of in situ REIC/Dkk-3 gene therapy for prostate cancer (2012)
  • Author(s): Kawauchi K, Watanabe M, Kaku H, Huang P, Sasaki K, Sakaguchi M, Ochiai K, Huh NH, Nasu Y, Kumon H
  • Journal Title: Acta Med Okayama Volume: 66(1) Pages: 7-16
  • Peer Reviewed
• [Journal Article] Tumor suppressor REIC/Dkk-3 interacts with the dynein light chain, Tctex-1 (2011)
  • Author(s): Ochiai K, Watanabe M, Ueki H, Huang P, Fujii Y, Nasu Y, Noguchi H, Hirata T, Sakaguchi M, Huh NH, Kashiwakura Y, Kaku H, Kumon H
  • Journal Title: Biochem Biophys Res Commun Volume: 412(2) Pages: 391-5
  • Peer Reviewed
• [Journal Article] Single Nucleotide Polymorphism WRN Leu1074Phe Is Associated with Prostate Cancer Susceptibility in Chinese Subjects (2011)
  • Author(s): Lei Wang, Haruki Kaku, Peng Huang, Kexin Xu, Kai Yang, Jiheng Zhang, Ming Li, Liping Xie, Xiaofeng Wang, Akiko Sakai, Masami Watanabe, Yasutomo Nasu, Kenji Shimizu, Hiromi Kumon, Yanqun Na
  • Journal Title: Acta Med Okayama Volume: (In press)
  • Peer Reviewed
• [Journal Article] Advanced two-step transcriptional amplification as a novel method for cancer-specific gene expression and imaging. (2011)
  • Author(s): Watanabe M, Ueki H, Ochiai K, Huang P, Kobayashi Y, Nasu Y, Sasaki K, Kaku H, Kashiwakura Y, Kumon H
  • Journal Title: Oncol Rep
  • Peer Reviewed
• [Journal Article] Implications of transcriptional factor, OCT-4, in human bladder malignancy and tumor recurrence (2011)
  • Author(s): Huang P, Chen J, Wang L, Na Y, Kaku H, Ueki H, Sasaki K, Yamaguchi K, Zhang K, Saika T, Nasu Y, Watanabe M, Kumon H
  • Journal Title: Med Oncol
  • Peer Reviewed
• [Journal Article] Tumor growth and metastasis suppression by Glipr1 gene-modified macrophages in a metastatic prostate cancer model. (2011)
  • Author(s): Tabata K, Kurosaka S, Watanabe M, Edamura K, Satoh T, Yang G, Abdelfattah E, Wang J, Goltsov A, Floryk D, Thompson TC
  • Journal Title: Gene Ther
  • Peer Reviewed
• [Book] REIC/Dkk-3 gene therapy (2011)
  • Author(s): Watanabe M, Kaku H, Huan g P, Kumon H, Nasu Y
  • Total Pages: 559-63
  • Publisher: Nihon Rinsho
• [Book] Suicide gene and immuno gene therapy for prostate cancer (2011)
  • Author(s): Nasu Y, Sasaki K, Kumon H, Kaku H, Watanabe M
  • Total Pages: 554-8
  • Publisher: Nihon Rinsho