A search for the interaction molecules with REIC/Dkk-3 in prostate gland.

2011 Fiscal Year Final Research Report

• Project/Area Number: 22791476
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Urology
• Research Institution: Nippon Veterinary and Life Science University (2011); The University of Tokushima (2010)
• Principal Investigator: OCHIAI Kazuhiko 日本獣医生命科学大学, 獣医学部, 助教 (30550488)
• Project Period (FY): 2010 – 2011
• Keywords: 癌 / 遺伝子 / REIC/Dkk-3

Research Abstract

REIC/Dkk-3 acts as a tumor suppressor in multiple cancer cell lines by inducing apoptosis through endoplasmic reticulum(ER) stress signaling. However, the intracellular interaction partners of REIC/Dkk-3 have not been fully elucidated. By employing yeast two-hybrid screening, we identified the human dynein light chain, Tctex-1, as a novel interaction partner of REIC/Dkk-3.The binding region of REIC/Dkk-3 with Tctex-1 contains an amino acid sequence motif [-E-X-G-R-R-X-H-] which was previously reported as the Tctex-1 binding domain of dynein intermediate chain(DIC). The link between REIC/Dkk-3 and Tctex-1 may be of significance for understanding the molecular functions of the proteins in ER stress signaling and intracellular dynein motor dynamics, respectively.

Research Products

• [Journal Article] Preclinical Safety and Efficacy of in Situ REIC/Dkk-3 Gene Therapy for Prostate Cancer (2012)
  • Author(s): Kawauchi, K., Watanabe, M., Kaku, H., Huang, P., Sasaki, K., Sakaguchi, M., Ochiai, K., Huh, NH., Nasu, Y., and Kumon, H.
  • Journal Title: Acta Med. Okayama Volume: 66(1) Pages: 7-16
  • URL: http://www.lib.okayama-u.ac.jp/www/acta/pdf/66_1_7.pdf
  • Peer Reviewed
• [Journal Article] Interactions between canine RAD51 and full length or truncated BRCA2 BRC repeats (2011)
  • Author(s): Ochiai, K., Yoshikawa, Y., Oonuma, T., Tomioka, Y., Hashizume, K., and Morimatsu, M.
  • Journal Title: Vet. J. Volume: 190(2011) Pages: 293-295
  • DOI: 10.1016/j.tvjl.2010.11.001
  • Peer Reviewed
• [Journal Article] Tumor suppressor REIC/Dkk-3 interacts with the dynein light chain, Tctex-1 (2011)
  • Author(s): Ochiai, K., Watanabe, M., Ueki, H., Huang, P., Fujii, Y., Nasu, Y., Sakaguchi, M., Huh, NH., Kashiwakura, Y., Kaku, H., and Kumon, H.
  • Journal Title: Biochem. Biophy. Res. Com Volume: 412(2) Pages: 391-395
  • DOI: 10.1016/j.bbrc.2011.07.109
  • Peer Reviewed
• [Journal Article] Advanced two-step transcriptional amplification as a novel method for cancer-specific gene expression and imaging (2011)
  • Author(s): Watanabe, M., Ueki, H., Ochiai, K., Huang, P., Kobayashi, Y., Nasu, Y., Sasaki, K., Kaku, H., Kashiwakura, Y., and Kumon, H.
  • Journal Title: Oncol. Rep. Volume: 26(4) Pages: 769-776
  • DOI: doi:10.3892/or.2011.1371
  • Peer Reviewed
• [Journal Article] Valine 1532 of human BRC repeat 4 plays an important role in the interaction between BRCA2 and RAD51 (2011)
  • Author(s): Ochiai, K., Yoshikawa, Y., Yoshimatsu, K., Oonuma, T., Tomioka, Y., Takeda, E., Arikawa, J., Mominoki, K., Omi, T., Hashizume, K., and Morimatsu, M.
  • Journal Title: FEBS Lett. Volume: 585(12) Pages: 1771-1777
  • DOI: 10.1016/j.febslet.2011.05.027
  • Peer Reviewed
• [Journal Article] Expression pattern of REIC/Dkk-3 in various cell types and the implications of the soluble form in prostatic acinar development. (2010)
  • Author(s): Zhang, K., Watanabe, M., Kashiwakura, Y., Li, SA., Edamura, K., Huang, P., Yamaguchi, K., Nasu, Y., Kobayashi, Y., Sakaguchi, M., Ochiai, K., Yamada, H., Takei, K., Ueki, H., Huh, NH., Li, M., Kaku, H., Na, Y., and Kumon, H.
  • Journal Title: Int. J. Oncol. Volume: 37(6) Pages: 1495-1501
  • DOI: doi:10.3892/ijo_00000802
  • Peer Reviewed
• [Presentation] イヌおよびヒトBRCA2のBRC repeat 3領域におけるミスセンス変異の比較 (2011)
  • Author(s): 吉川泰永、森松正美、落合和彦、折野宏一、相田成一、垰田高広、近澤征史朗、岩井聡美、渡辺清隆
  • Organizer: 第34回日本分子生物学会年会
  • Year and Date: 2011-12-15
• [Presentation] イヌ乳腺腫瘍関連遺伝子産物BRCA2のBRC repeat 4のアミノ酸がDNA相同組換え修復に必須なRAD51ホモ会合体形成に与える影響 (2011)
  • Author(s): 落合和彦、吉川泰永、近江俊徳、森松正美
  • Organizer: 第152回日本獣医学会学術集会
  • Year and Date: 2011-09-20
• [Presentation] イヌにおけるヒトABO組織血液型遺伝子に相同なfull length cDNAの同定と特徴 (2011)
  • Author(s): 近江俊徳、鄭英和、市東正幸、宇田川智野、河上剛、落合和彦、杉山将、土田修一
  • Organizer: 第152回日本獣医学会学術集会
  • Year and Date: 2011-09-20
• [Presentation] 癌抑制遺伝子BRCA2のPCR法による変異解析法の確立 (2011)
  • Author(s): 吉川泰永、森松正美、鈴木優、西村百合香、落合和彦、折野宏一、垰田高広、近澤征史朗、岩井聡美、島村麻子、新井敏郎、渡辺清隆
  • Organizer: 第152回日本獣医学会学術集会
  • Year and Date: 2011-09-20
• [Patent(Industrial Property Rights)] REIC/Dkk-3タンパク質の部分領域ポリペプチド (2011)
  • Inventor(s): 公文裕巳、渡部昌実、二見淳一郎、藤井康之、植木英雄、落合和彦
  • Industrial Property Rights Holder: 国立大学法人岡山大学
  • Industrial Property Number: 国際出願、PCT/JP2011/014319
  • Filing Date: 2011-07-01
  • Overseas