Establishment of new prostate cancer metastatic model with androgen independent growth and identification of new mechanism of androgen-independency

2011 Fiscal Year Final Research Report

• Project/Area Number: 22791487
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Urology
• Research Institution: Nagoya City University
• Principal Investigator: NAIKI Taku 名古屋市立大学, 大学院・医学研究科, 臨床研究医 (50551272)
• Project Period (FY): 2010 – 2011
• Keywords: 去勢抵抗性前立腺癌 / 転移動物モデル / 分子標的治療

Research Abstract

We established androgen-independent prostate cancer cell line from TRAP tumor, and named PCai1, and previously reported the orthotopic metastatic models, and tail vein injection model using PCai1 cells. We investigate the GST-P expression of PCai1 by RT-PCR and Western blot. In addition, using GST-P-siRNA, we investigated the relationship between the cell growth and Reactive Oxygen Species(ROS) in PCai1.PCai1 cells had higher GST-P expression in mRNA and protein levels in Charcoal Stripped-FBS medium than in normal medium. GST-P knocked down by siRNA resulted in significant decrease of the proliferation rate in vitro. On the contrary, DCFH assay revealed that ROS was induced by GST-P-siRNA treat. Tumors metastasized in the bone marrow after intravenous injection expressed high levels of GST-P, while metastatic lesions in the lung and lymph nodes failed to express GST-P as seen in the case of the orthotopic implantation. GST-P might have the important roles in the prostate cancer growth and metastasis by the restriction of ROS.

Research Products

• [Journal Article] Organ specific Gst-pi expression of the metastatic androgen independent prostate cancer cells in nude mice (2011)
  • Author(s): Naiki T, Asamoto M, Toyoda-Hokaiwado N, Naiki-Ito A, Tozawa K, Kohri K, Takahashi S, Shirai T
  • Journal Title: The Prostate
  • Peer Reviewed
• [Journal Article] Preoperative Prediction of Neurovascular Bundle Involvement of Localized Prostate Cancer by Combined T2 and Diffusion-weighted Imaging of Magnetic Resonance Imaging, Number of Positive Biopsy Cores, and Gleason Score (2011)
  • Author(s): Naiki T, Okamura T, Nagata D, Mori Y, Kawai N, Ogawa K, Akita H, Hashimoro Y, Tozawa K, Kohri K
  • Journal Title: Asian Pac J Cancer Prev Volume: 12 Pages: 909-13
  • Peer Reviewed
• [Journal Article] Tranilast suppresses prostate cancer growth and differentiation in vivo and in vitro (2010)
  • Author(s): Sato S, Takahashi S, Asamoto M, Naiki T, Naiki-Ito A, Asai K, Shirai T
  • Journal Title: The Prostate Volume: 70 Pages: 229-38
  • Peer Reviewed
• [Presentation] Glutathione oxidation-reduction system facilitates androgen independent prostate cancer growth (2011)
  • Author(s): Naiki T, Asamoto M, Naiki A, Kawai N, Tozawa K, Kohri K
  • Organizer: 第70回日本癌学会総会
  • Place of Presentation: 名古屋市
  • Year and Date: 20111003-05
• [Presentation] 再燃前立腺癌における酸化ストレス応答因子の役割 (2011)
  • Author(s): 内木拓, 朝元誠人, 内木綾, 小島祥敬, 河合憲康, 戸澤啓一, 白井智之, 郡健二郎
  • Organizer: 第99回日本泌尿器科学会総会
  • Place of Presentation: 名古屋市
  • Year and Date: 20110421-24
• [Presentation] Metastatic site specific different expression of GST-P in new androgen independent prostate cancer cells from TRAP model (2010)
  • Author(s): Naiki T, Asamoto M, Naiki-Ito A, Tozawa K, Kohri K, Shirai T
  • Organizer: 第69回日本癌学会学術総会シンポジウム
  • Place of Presentation: 大阪市
  • Year and Date: 20100922-24
• [Presentation] ラット前立腺癌モデル由来細胞株によるアンドロゲン非依存性前立腺癌転移モデルの確立 (2010)
  • Author(s): 内木拓, 朝元誠人, 内木綾, 戸澤啓一, 白井智之, 郡健二郎
  • Organizer: 第98回日本泌尿器科学会総会
  • Place of Presentation: 盛岡市
  • Year and Date: 20100427-30