2011 Fiscal Year Final Research Report
Establishing orthotopic model of prostate cancer metastasis to bone in mice
| Project/Area Number |
22791497
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Urology
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| Research Institution | Keio University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Keywords | 前立腺癌 / 骨転移モデル |
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| Research Abstract |
TRAMP-C1 is derived from a prostate adenocarcinoma in transgenic C57BL/6 mice. TRAMP-C1/CBR cells were genetically modified to express the luciferase gene. Nude mice(BALB/c, nu/nu) were anesthetized and received injection of TRAMP-C1/CBR cells with/without mesenchymal stem cells(MSC) in the prostatic lobe through a midline lower abdominal incision. To detect cancer development in mice, we used bioluminescence imaging. In orthotopic model, we generally detected a hot spot of bioluminescence in lower abdomen of mice. By the combination injection of TRAMP-C1 and MSC, the local hot spot was remarkably enlarged, however, we could not confirm the hot spot to prove the bone metastasis.
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