2011 Fiscal Year Final Research Report
Regeneration of oocytes from frozen ovaries : establishment of novel method for efficient pregnancy
| Project/Area Number |
22791512
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Akita University |
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Principal Investigator |
KUMAZAWA Yukiyo 秋田大学, 大学院・医学系研究科, 助教 (70400504)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 原始卵胞 / 卵胞発育 / PTEN / PI3K / ゲノムインプリンティング |
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| Research Abstract |
In this study, we could activate dormant primordial follicles following the in vitro treatment of ovarian fragments to stimulate PI3K/PKB signaling system. The transplantation of these cultured ovarian fragments allowed to grow the activated primordial follicles to preovulatory stage. We could retrieve mature oocytes from the preovulatory follicles and normal pups were delivered after in vitro fertilization and embryo transfer. This method is expected to develop a novel method for pregnancy by using autologous ooytes in patients who lack follicle development but have residual primordial follicles.
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