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2011 Fiscal Year Final Research Report

Analysis of functional role of intereukin-6 secreted by tumor-associated macrophage in ovarian cancer ascites

Research Project

  • PDF
Project/Area Number 22791529
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionOsaka University

Principal Investigator

ISOBE Aki  大阪大学, 大学院・医学研究科, 助教 (60397619)

Project Period (FY) 2010 – 2011
Keywords卵巣癌 / 腹膜播種 / TAM / IL-6 / tocilizumab
Research Abstract

Ascites were collected aseptically, and CD11b positive cells(TAMs) were purified by positive selection using magnetic-activate cell sorting(MACS) technology. Co-culture of ovarian cancer cell line, SKOV3ip1 cells, with TAMs significantly enhanced the invasive activity of cancer cells in Matrigel Boyden chamber assay. This enhancement was partially blolcked by the treatment of anti-interleukin(IL)-6 receptor antibody(Tocilizumab). Since SKOV3ip1 cells did not express IL-6 in mRNA and protein level, IL-6 secreted by TAMs were considered to play a key role in ovarian cancer invasion. In vivo ovarian cancer xenografts, i. p. treatment of Tocilizumab significantly reduced peritoneal dissemination and ascites formation. Those results suggested targeting IL-6 produced by TAMs could be a promising therapy for a subset of ovarian cancer patients.

  • Research Products

    (3 results)

All 2011 2010

All Presentation (3 results)

  • [Presentation] 卵巣がん進展時の腹水中に大量に存在する骨髄由来細胞が果たす役割の解明2011

    • Author(s)
      磯部晶.澤田健二郎.大八木知史.若林敦子.馬淵誠士.森重健一郎.木村正
    • Organizer
      第63回日本産科婦人科学会
    • Place of Presentation
      大阪
    • Year and Date
      2011-08-29
  • [Presentation] Analysis of functional role of interleukin-6 secreted by tumor-associated macrophages in ovarian cancer ascites2011

    • Author(s)
      Isobe, A. Sawada, K. Ohyagi, C. Mabuchi, S. Wakabayashi, A. Morishige, K. Kimura, T
    • Organizer
      American Association for Cancer Research 102^<nd> Annual Meeting 2011
    • Place of Presentation
      Orland, USA
    • Year and Date
      2011-04-05
  • [Presentation] Analysis of functional role of interleukin-6 secreted by tumor-associated macrophages in ovarian cancer ascites2010

    • Author(s)
      Isobe, A. Sawada, K. Ohyagi, C. Mabuchi, S. Kimura, T
    • Organizer
      第69回日本癌学会
    • Place of Presentation
      大阪
    • Year and Date
      2010-09-24

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Published: 2013-07-31  

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