2011 Fiscal Year Final Research Report
DNA methylation profile of tissue dependent and differentially methylated region in human estrogen receptor-alpha promoter region
| Project/Area Number |
22791534
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Yamaguchi University |
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Principal Investigator |
ASADA Hiromi 山口大学, 医学部附属病院, 助教 (90526906)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 生殖医学 |
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| Research Abstract |
In upstream promoter region of ERαgene, DNA methylation status was unmethylated in tissues with high ERαmRNA levels, endometrium and mammary gland, and methylated in tissues with low ERαmRNA levels, placenta and skin. Using the reporter assay, we demonstrated that DNA methylation at the upstream region of ERαreduced the promoter activity by about 20%. We suggest the upstream region of ERαis tissue dependent and differentially methylated region(T-DMR).
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