Proteomics-based exploration of signaling molecules responsible for human endometrial function

2011 Fiscal Year Final Research Report

• Project/Area Number: 22791547
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Obstetrics and gynecology
• Research Institution: Keio University
• Principal Investigator: NISHIKAWA Sayaka 慶應義塾大学, 医学部, 助教 (60445236)
• Project Period (FY): 2010 – 2011
• Keywords: 子宮内膜 / シグナル伝達 / 脱落膜化 / 着床 / キナーゼ

Research Abstract

The aim of this research project was to explore the signaling molecules and pathways particularly involving SRC responsible for human endometrial function using proteomics methods. We attempted to construct an adenovirus vector expressing constitutively active SRC that was required as an essential material to accomplish this project, but we did not succeeded yet. Instead, we successfully prepared several research materials and tools such as reporter constructs, an immortalized human endometrial cell line, and in vivo model of human endometrial reconstitution. We also demonstrated that epithelial mesenchymal transition(EMT) involving N-cadherin played a role in human endometrial epithelial cell function.

Research Products

• [Journal Article] Studies using an in vitro model show evidence of involvement of epithelial-mesenchymal transition of human endometrial epithelial cells in human embryo implantation (2012)
  • Author(s): Uchida H, Maruyama T, Nishikawa-Uchida S, Oda H, Miyazaki K, Yamasaki A, Yoshimura Y
  • Journal Title: J Biol Chem Volume: 287(7) Pages: 4441-4450
  • Peer Reviewed
• [Journal Article] OCT4 expression in human uterine myometrial stem/progenitor cells (2010)
  • Author(s): Ono M, Kajitani T, Uchida H, Arase T, Oda H, Nishikawa-Uchida S, Masuda H, Nagashima T, Yoshimura Y, Maruyama T
  • Journal Title: Hum Reprod Volume: 25(8) Pages: 2059-2067
  • Peer Reviewed
• [Presentation] 流産検体のアレイCGH解析により不育症夫婦に均衡型微細相互転座が判明した一例 (2011)
  • Author(s): 丸山哲夫, 小澤伸晃, 会津義紀, 内田明花, 各務真紀, 宮崎薫, 小田英之, 荒瀬透, 内田浩, 吉村泰典
  • Organizer: 第56回日本人類遺伝学会
  • Place of Presentation: 千葉県千葉市・幕張メッセ
  • Year and Date: 20111109-12
• [Presentation] Contribution of myometrial stem/progentior cells to pregnancy-induced uterine remodeling (2011)
  • Author(s): Masanori Ono, Tetsuo Maruyama Takashi Kajitani, Hiroshi Uchida Hideyuki Oda, Sayaka Uchida, Toru Arase, Akiko Yamasaki, Takashi Nagashima, Hirotaka Masuda, Yasunori Yoshimura
  • Organizer: 2nd World Congress on Reproductive Biology(WCRB)
  • Place of Presentation: Cairns, Australia
  • Year and Date: 20111009-12
• [Presentation] Possible evidence for epithelial-mesenchymal transition mediating displacement of endometrial cells away from the site of embryo implantation (2011)
  • Author(s): Hiroshi Uchida, Tetsuo Maruyama, Sayaka Uchida-Nishikawa, Yasunori Yoshimura
  • Organizer: 2nd World Congress on Reproductive Biology(WCRB)
  • Place of Presentation: Cairns, Australia
  • Year and Date: 20111009-12
• [Presentation] 多嚢胞性卵巣腫大を反復する患者に見いだされた変異FSH受容体の機能解析 (2011)
  • Author(s): 西川明花, 丸山哲夫, 内田浩, 各務真紀, 小田英之, 宮崎薫, 青木大輔, 吉村泰典
  • Organizer: 第63回日本産科婦人科学会
  • Place of Presentation: 大阪府大阪市・大阪国際会議場
  • Year and Date: 20110829-31
• [Presentation] Possible involvement of UDP-glucose and its recepter P2RY14 in embryo implantation (2011)
  • Author(s): Toru Arase, Tetsuo Maruyama, Hiroshi Uchida, Kaoru Miyazaki, Hideyuki Oda, Sayaka Uchida-Nishikawa, Maki Kagami, Akiko Yamasaki, Kayoko Tamaki, Yasunori Yoshimura
  • Organizer: 27th European Society of Human Reproduction & Embryology(ESHRE)
  • Place of Presentation: Stockholm, Sweden
  • Year and Date: 20110703-06
• [Presentation] CD93 and CD31 as possible candidate markers for human endometrial stem/progenitor cells (2011)
  • Author(s): Kaoru Miyazaki, Takashi Kajitani, Hirotaka Masuda, Toshio Hamatani, Hideyuki Oda, Akiko Yamasaki, Sayaka Nishikawa-Uchida Hiroshi Uchida, Yasunori Yoshimura, Tetsuo Maruyama
  • Organizer: 9th International Society for Stem Cell Research(ISSCR)
  • Place of Presentation: Tronto, Ontario Canada
  • Year and Date: 20110615-18
• [Presentation] ヒト着床における子宮内膜上皮細胞動態.卵巣ホルモンと低分子Gタンパク質の関与- (2011)
  • Author(s): 内田浩, 丸山哲夫, 西川明花, 小田英之, 宮崎薫, 各務真紀, 吉村泰典
  • Organizer: 第84回日本内分泌学会
  • Place of Presentation: 兵庫県神戸市・神戸国際会議場
  • Year and Date: 20110421-23
• [Presentation] Possible involvement of endometrial stem/progenitor cells in the pathogenesis of endocetriosis (2010)
  • Author(s): Tetsuo Maruyama, Hirotaka Masuda, Takashi Nagashima, Takashi Kajitani, Masanori Ono, Sayaka Nishikawa-Uchida Hideyuki Oda, Kaoru Miyazaki, Toru Arase, Maki Kagami, Hiroshi Uchida Hironori Asada, Yasunori Yoshimura
  • Organizer: 1st Asian Conference on Endometriosis(ACE)
  • Place of Presentation: Shanghai, China
  • Year and Date: 20101016-17
• [Presentation] 自然発症した卵巣過剰刺激症候群症例におけるFSH受容体の遺伝子変異とその機能解析 (2010)
  • Author(s): 西川明花, 丸山哲夫, 内田浩, 小田英之, 各務真紀, 宮崎薫, 青木大輔, 吉村泰典
  • Organizer: 第62回日本産科婦人科学会
  • Place of Presentation: 東京都千代田区・東京国際フォーラム
  • Year and Date: 20100423-25
• [Presentation] Molecular Analysis of Mutated FSH Recepter Detected in a Patient with Spontaneous Ovarian Hyperstimulation Syndorome (2010)
  • Author(s): Sayaka Nishikawa-Uchida, Tetsuo Maruyama, Hiroshi Uchida, Takashi Kajitani, Hideyuki Oda, Kaoru Miyazaki, Maki Kagami, Yasunori Yoshimura
  • Organizer: 57th Society for Gynecologic Investigation(SGI)
  • Place of Presentation: Orlando, Florida, USA
  • Year and Date: 20100324-27