2011 Fiscal Year Final Research Report
The maintenance of the IgE antibody secreting cells
| Project/Area Number |
22791572
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Chiba University |
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Principal Investigator |
INAMINE Ayako 千葉大学, 大学院・医学研究院, 特任研究員 (70466720)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 鼻科学 / 免疫学 |
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| Research Abstract |
In seasonal allergy (such as Japanese cedar pollinosis), pollen specifi c IgE Ab production has been persistent even in the absent season of allergens. Since long-lived plasma cells (LL-PCs) in bone marrow (BM) are important for the maintenance of serum Ab titers, the persistent production of IgE Abs in Allergic patients may be due to IgE-LL-PCs in BM. However, IgE-LL-PCs in murine BMs have never been detected. Moreover, high concentration of IgE Abs was detected in serum of nasal polyposis patients and IgE-PCs were accumulated in the nasal polyposis tissue. In this study, we investigated the mechanism of IgE-LL-PCs generation using IL-21R-KO mice which have high concentration of serum IgE titers. When the number of IgE-PCs was examined in IL-21R-KO mice by ELISPOT assay, no IgE-PCs were detected in BM, despite of the high serum IgE concentration. Then, we have established the culture system to generate LL-PCs from naive B cells using IL-21, and those IgE-PCs were transferred into mice to examine the generation of IgE-LL-PCs in BMs. Since LL-PCs are derived from germinal center (GC) B cells was impaired in IL-21R-KO mice, we will discuss the role of IL-21 in the generation of LL-PCs including IgE-LL-PCs. These results suggested that IgE-LL-PCs would provide a new target for immunotherapy in Allergic diseases.
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[Journal Article] Increase of regulatory T cells and the ratio of specific IgE to total IgE are candidates for response monitoring or prognostic biomarkers in two-year sublingual immunotherapy (SLIT) for Japanese cedar pollinosis2011
Author(s)
Fujimura, T., Yonekura, S., Horiguchi, S., Taniguchi, Y., Saito, A., Yasueda, H., Inamine, A., Nakayama, T., Takemori, T., Taniguchi, M., Sakaguchi, M., Okamoto, Y.
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Journal Title
Clin. Immunol
Volume: 139(1)
Pages: 65-74
Peer Reviewed
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