2012 Fiscal Year Final Research Report
Functional analysis of the ubiquitin -proteasome system in inner ear.
| Project/Area Number |
22791586
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Shinshu University |
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Principal Investigator |
KITO Ryosuke 信州大学, 医学部附属病院, 助教(特定雇用) (80419358)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 耳科学 / 内耳 / ユビキチン / プロテアソーム |
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| Research Abstract |
Ubiquitin-Proteasome system was a important phenomena including protein degradation, DNA repair, translational control and signal transduction. In our cDNA microarray analysis of the inner ear, UbA52 protein was highly expressed than other tissues. But the functional roll of the UbA52 protein in the inner ear did not known. In this study we tried to investigate the functional roll of the UbA52 protein in the inner ear, we performed 2D gel electrophoresis and western blot, and found 4 significant protein targets. After MS/MS analysis, the 2 of these protein were cysteine sulfinic acid decarboxylase (CSAD) and aldehyde dehydrogenase (ALDH).
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