2011 Fiscal Year Final Research Report
Comprehensive profiling of the gene expression in the inner ear sensory epithelium and its application to the regeneration
| Project/Area Number |
22791595
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Keywords | 遺伝子発現解析 / 内耳 / Notchシグナル / Septin / プロスタグランジン |
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| Research Abstract |
We performed preliminary experiments to set up the condition of extracting RNA from single cochlear cells and amplifying the cDNA linearly. In addition, we found the function of several genes in the cochlea. These results will contribute to the realization of cochlear regenerative medicine. Complete inhibition of Notch signaling in the mouse cochlear caused small numbers of inner hair cells only in the apical turn. This result indicates that Notch signaling is involved not only in the determination of cell fate but also in the maintenance of the cochlear sensory epithelial progenitors. The analysis of EP4 knockout mice revealed that prostaglandin E physiologically protects cochlear hair cells. We also found the new markers of cochlear supporting cells, Septin4, Septin5, and Septin7.
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Research Products
(14 results)
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[Journal Article] Topicalinsulin-like growth factor 1 treatment using gelatin hydrogels for glucocorticoid-resistant sudden sensorineural hearing loss : a prospective clinical trial2010
Author(s)
Nakagawa T, Sakamoto T, Hiraumi H, Kikkawa YS, Yamamoto N, Hamaguchi K, Ono K, Yamamoto M, Tabata Y, Teramukai S, Tanaka S, Tada H, Onodera R, Yonezawa A, Inui K, Ito J
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DOI
Peer Reviewed
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