2012 Fiscal Year Final Research Report
Acceleration of photoreceptor induction from human iPSCs with gene transfer.
| Project/Area Number |
22791702
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Ophthalmology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
OKAMOTO Satoshi 独立行政法人理化学研究所, 網膜再生医療研究開発プロジェクト, リサーチアソシエイト (50509106)
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| Project Period (FY) |
2010 – 2012
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| Keywords | iPS 細胞 / ES 細胞 / 分化誘導 / 網膜 |
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| Research Abstract |
I investigated the possibility of gene transfer in promoting effect in photoreceptor differentiation in order to shorten the culture period of photoreceptor induction from ESCs/iPSCs. Despite various examinations, the original purpose was pending, however, in the investigation process, Nrl-EGFP mouse iPSCs that expressed EGFP in photoreceptors specifically were produced to detect the effect of gene transfer, and the effect of an inhibitor in retinoic acid signaling to elevate the efficiency of retinal progenitor induction was developed. These research products have expected to work as powerful research tools in other studies of retinal regenerative medicine.
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