2012 Fiscal Year Final Research Report
Study ofthe molecular mechanisms of delayed wound healing by using klothomice,which have phenotypessimilar to skin ulcerof aged humans.
| Project/Area Number |
22791726
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Plastic surgery
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
YAMASHITA Ken 札幌医科大学, 医学部, 助教 (30312507)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 外科 / 老化 / 創傷治癒 / 皮膚潰瘍 / klothoマウス |
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| Research Abstract |
The aim of this study was to analyze the molecular mechanisms of delayed wound healing with agingby using α-klotho-deficient (klotho) mice,which have phenotypessimilar to those of aged humans.Wound healing time, histology and mRNA expression were investigated using a skin defect wound model of klothomice. Theklotho mice showed delayed wound healingandimpairedgranulation formation compared to those in wild-type (WT) mice.The reverse transcript polymerase chain reaction (RT-PCR)analysis revealed enhancement of the mRNA expression of interleukin(IL)-1β, IL-6and tumor necrosis factor (TNF)-α, and depressionof laminin-5 in skin wound lesions of klotho mice compared to the levels in WT mice.
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