2012 Fiscal Year Final Research Report
The research of the cell reaction to the different bacterial infection
| Project/Area Number |
22791762
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2012
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| Keywords | 口腔細菌学 |
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| Research Abstract |
P. gingivalis are invaginated by gingival epithelial cells via the endocytic pathway, and some intracellular bacteria are sorted to lytic compartments, including autolysosomes and late endosomes/lysosomes, while a considerable number of the remaining organisms are sorted to recycling endosomes, followed by bacterial exit from the cells. Exited bacteria can re-enter fresh cells. This result strongly suggests that the recycling pathway is exploited by gingivalis to exit from infected cells to neighbouring cells as intracellular P. a mechanism of cell-to-cell spreading.Knockdown of the combinational SNARE proteins Vti1b and VAMP8 with siRNAs disturbs the fusion of GcAV (GAS containing Autophagosome like Vacuole) with lysosome. This finding indicates that VAMP8 and Vti1b mediate fusion with lysosomes in antimicrobial autophagy.
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