2011 Fiscal Year Final Research Report
Clarification of the disintegration of Hertwig's epithelial root sheath by using a real time imaging
| Project/Area Number |
22791774
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Iwate Medical University |
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Principal Investigator |
OTSU Keishi 岩手医科大学, 歯学部, ポストドクター (60509066)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 歯根 / 発生 / Hertwig's上皮鞘 / EMT / ROCK |
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| Research Abstract |
Tooth root formation is initiated by the development of Hertwig's epithelial root sheath(HERS) from the cervical loop in the enamel organ. However, the cellular dynamics during HERS formation is not well known yet. In This study, we aimed to establish the experimental system to visualize the dynamics of HERS cells in order to elucidate the mechanism of HERS disintegration by EMT. We established the real time slice culture imaging system, which could observe the migration, division, and actin promoter activity in HERS cell. We also established HERS cell line from mouse molars. The cells expressed mesenchymal cell markers, and the expressions were increased to the stimulation of TGF-β. The root elongation was intensified by HGF with the increased cell proliferation, indicating the implication between HGF signaling and tooth root formation.
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Research Products
(33 results)
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[Journal Article] Histological analysis of epithelial stem cells during induced pluripotent stem cell-derived teratoma development2012
Author(s)
Kishigami, R., Otsu, K., Oikawa-Sasaki, A., Fujiwara, N., Ishizeki, K., Tabata, Y., Harada, H.
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Journal Title
J. Oral Biosci
Volume: 54
Pages: 54-58
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[Journal Article] Differentiation of induced pluripotent stem cells into dental mesenchymal cells2012
Author(s)
Otsu, K., Kishigami, R., Oikawa-Sasaki, A., Fukumoto, S., Yamada, A., Fujiwara, N., Ishizeki, K. Harada, H.
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Journal Title
Stem Cells & Dev
Volume: (in press)
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[Journal Article] Role of epithelial-stem cell interactions during dental cell differentiation2012
Author(s)
Arakaki M, Ishikawa M, Nakamura T, Iwamoto T, Yamada A, Fukumoto E, Saito M, Otsu K, Harada H, Yamada Y, Fukumoto S
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Journal Title
J. Biol. Chem
Volume: 287
Pages: 10590-10601
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[Journal Article] Establishment of Hertwig's epithelial root sheath cell line from cells involved in epithelial-mesenchymal transition2011
Author(s)
Akimoto, T., Fujiwara, N., Kagiya, T., Otsu, K., Ishizeki, K., Harada, H.
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Journal Title
BBRC
Volume: 404
Pages: 308-312
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[Journal Article] Hepatocyte growth factor stimulates root growth during the development of mouse molar teeth2011
Author(s)
Sakuraba, H., Fujiwara, N., Sasaki-Oikawa, A., Sakano, M., Otsu, K., Ishizeki, K., Harada, H.
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Journal Title
J. Period. Res
Volume: 47
Pages: 81-88
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