2011 Fiscal Year Final Research Report
Examination of an adjuvant effect to the cancer cell of CpG in vivo
| Project/Area Number |
22791953
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Surgical dentistry
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| Research Institution | Akita University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Keywords | がん |
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| Research Abstract |
(2010)(1) B16 melanoma were purchased from RIKEN, incubated for proliferation. (2) Anticancer drug, 5-FU were administered to WT mice(C57BL/6), and myelosuppression was observed, so called nadir, at 7days Flowcytometry and HE staining. (3) An adjuvant CpG were administered, the production of inflammatory cytokine were observed by ELISA. (2011) (1) B16 melanoma were inoculated s.c., as a result, confirmed engrafted model. Myeloid derived suplessive cells(MDSC) resident in peripheral blood at 8%, but 7days after 5-FU injection, MDSC were decreased at 4%. On the other, 5-FU and CpG combined injected, MDSC were increased at 20%.
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