2011 Fiscal Year Final Research Report
Investigation on the formation of immune privilege in tissue-engineered cartilage and its application on cartilage regenerative medicine
| Project/Area Number |
22791956
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Surgical dentistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
FUJIHARA Fujihara 東京大学, 医学(系)研究科(研究院), 特任助教 (50466744)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 再生医療 / 再生軟骨 / 免疫特権 |
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| Research Abstract |
Involvement of immune privilege in the transplantation of tissue-engineered cartilage was examined in mice. FasL and TGF-βwere expressed on chondrocytes of tissue-engineered cartilage constructs two weeks after transplantation. The constructs of FasL-hypomorphic mice(gld) showed more infiltration of F4/80-positive cells with less accumulation of proteoglycan than those of wild-type mice. When gld chondrocytes were cultured with macrophage-like cell, RAW264, they showed modest cytotoxicity and less induction of apoptosis on RAW264 than those of wild-type mice, suggesting that expression of FasL on chondrocytes could modulate the viability and localization of macrophages, promoting the maturation of tissue-engineered cartilage.
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