2011 Fiscal Year Final Research Report
Development of an invitro keratinocyte cell culture model for analysis of mechanisms of Bisphosphonate-related osteonecrosis of the jaws(BRONJ)
| Project/Area Number |
22791963
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Surgical dentistry
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| Research Institution | Niigata University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Keywords | ビスフスフォネート(BP) / BP関連顎骨壊死 / 病因 / 口腔粘膜上皮 / 細胞周期 |
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| Research Abstract |
Bisphosphonates(BP) causes bisphosphonate-related osteonecrosis of the jaw(BRONJ). While the etiology of BRONJ has not been clarified, it would appear that the compromised oral mucosa wound healing is associated with the onset of BRONJ. The effects of BPs on the oral mucosa keratinocytes were examined by 2D as well as 3D oral keratinocyte culture system. In consequence, we found BP induced DNA damage response in the oral mucosa keratinocytes and caused cell cycle arrest in S-phage, indication the inhibitors of the cell proliferation. Thus, it is suggested that BPs are related to the onset of BRONJ by a loss of oral mucosa tissue integrity.
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