2011 Fiscal Year Final Research Report
The identification of the target protein of N-α-acetyltransferase and the devel opment of a newly therapy by the control of N-α-acetylome
| Project/Area Number |
22791976
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Surgical dentistry
|
|---|
| Research Institution | Tottori University |
|---|
Principal Investigator |
TAKUBO Kazuko 鳥取大学, 医学部附属病院, 助教 (90379628)
|
|---|
| Project Period (FY) |
2010 – 2011
|
| Keywords | アセチローム / αアセチル化 / 細胞死 / 5-FU |
|---|
| Research Abstract |
I found out that 5-FU the decreases mRNA and the protein revel of N-α-acetyltransferase(NATH) in laryngeal cancer origin HEp-2. It is suggested that the control of acetylome by NATH is an important factor for regulation of cancer cells. The aim of this study is to develop of a newly therapy based on control of acetylome.
The research results are as follows. In order to identify NATH target protein, protein was collected from HEp-2 which were transfected with siNATH and the negative control siRNA(siNC) and Western blot was done in two protein(HSP70, HSP90) which was admitted downregulation in proteomic analysis and TS which was admitted the downregulation of mRNA expression. However, the difference of the amount of protein revel was not accepted in siNATH group and siNC group in Western blot.
|
