2011 Fiscal Year Final Research Report
The role of FGF binding protein HBp17 in cancer stem cells of oral cancer and application for the molecular target therapy.
| Project/Area Number |
22791982
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Surgical dentistry
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Keywords | 増殖因子 / ビタミンD3 |
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| Research Abstract |
By assuming the expression of HBp17 in an oral cancer cell line, we treated them with 1α, 25(OH)_2D_3(VD_3). We investigated the expression of HBp17, FGF-2 and NF-κB molecules. Reporter assays were done for proof of down-regulation of these molecules.
The HBp17 expression was down-regulated with VD3. There are no changes in NF-κB expression(p65 or p50). However the expression of IκB-α was increased and its phosphorylation was decreased. Investigation on the HBp17 promoter activity by luciferase reporter assays showed the down-regulation of this molecule by VD3. We found that HBp17 expression in nuclear decreased by VD3 treatment with immunofluorescence cytochemistry. We concluded that VD3 down-regulated HBp17 expression by inhibiting DNA binding of NF-κB.
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