2011 Fiscal Year Final Research Report
Molecular diagnosis of sentinel lymph node metastases for intraoperative detection in oral cancer
| Project/Area Number |
22791988
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Surgical dentistry
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| Research Institution | Ehime University |
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Principal Investigator |
GODA Hiroyuki 愛媛大学, 医学部附属病院, 助教 (00464371)
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| Project Period (FY) |
2010 – 2011
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| Keywords | センチネルリンパ節 / 分子診断 / OSNA法 |
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| Research Abstract |
Sixty-one of 312 lymph nodes were pathologically metastasis-positive. The overall concordance rate between the OSNA assay using breast cancer criteria and histopathology was 94. 2%. The optimal cut-off for the copy number of CK19 mRNA in assessing lymph node metastasis of HNSCC was 300 copies/ll, which had the highest diagnostic accuracy(95. 2%). The OSNA assay can be completed within 30 min. And we identified ANXA8L2 and DSG3 markers by microarray analysis. These markers diagnosed metastatic lymph nodes in 100% sensitivity.
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