2011 Fiscal Year Final Research Report
Metabolisms of extracellular matrix in temporomandibular joint
| Project/Area Number |
22792002
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Surgical dentistry
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| Research Institution | Kyushu Dental College |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Keywords | 顎関節 / 細胞外マトリックス / アグリカナーゼ / ヒアルロン酸 |
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| Research Abstract |
Hyaluronan(HA) oligosaccharides serve as competitive receptor antagonists to displace HA from the cell surface and induce cell signaling events. We determined changes in the expression and function of aggrecanases after disruption of chondrocyte CD44.HA interactions. Disruption of chondrocyte CD44.HA interactions with HA oligosaccharides induced the transcription of ADAMTS-4 and ADAMTS-5.The association of GPI. anchored MT4-MMP with ADAMTS-4 was also induced in articular chondrocytes by HA oligosaccharides. Inhibition of the NF-kappa B pathway blocked HA oligosaccharides-mediated stimulation of aggrecanases. These results suggest that disruptive changes in chondrocyte. matrix interactions by HA oligosaccharides induce matrix degradation and elevate aggrecanases via the activation of the NF-kappa B signaling pathway.
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