2011 Fiscal Year Final Research Report
Development of next-generation therapy for temporomandibular joint arthrosis by gene transfer technique using nanofullerene and ultrasonic wave
| Project/Area Number |
22792004
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Surgical dentistry
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| Research Institution | Kyushu Dental College |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Keywords | ソノポレーション / フラーレン / ナノバブル / 遺伝子導入 |
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| Research Abstract |
We develop the next-generation therapy for temporomandibular joint arthrosis by gene transfer techmique using the nanofullerene and ultrasonic wave. HIG-82 cells were seeded into silicon chambers. The chambers were attached to a stretching apparatus which applied a uniaxial sinusoidal stretching force. The amounts of IL-1β, IL-1ra, PGE2 released into the culture medium were determined using ELISA. Nanofullerene pretreatment suppressed PGE2 synthesis induced by mechanical stretch. We used sonoporation to transfect DNA-nanofullerene into HIG-82 cells and examined the transfection efficiency. Transfection efficiency was significantly increased when the cells were ultrasonicated with nanofullerene.
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