2011 Fiscal Year Final Research Report
Analysis of structural abnormality of whole chromosomes and novel tumor suppressor gene using mapping arrays in oral cancer
| Project/Area Number |
22792017
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Surgical dentistry
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| Research Institution | Tokyo Dental College |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Keywords | 口腔がん / DNAマッピングアレイ / ヘテロ接合性消失(LOH) |
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| Research Abstract |
[Purpose] We analyzed genomic copy number abnormality on all genomes in the oral squamous cell carcinoma using Affymetrix GeneChip^<(R)> Mapping 10K Array in this study and tried the identification of the new metastasis connection gene of the oral cavity squamous cell carcinoma. [Materials and Methods] We used 20 oral squamous cell carcinoma cases. We analyzed a metastasis to regional lymph node-positive case(pN(+)) 3 case and a negative case(pN(-)) 2 case by GeneChip(R) Mapping 10K Array for histopathology and detected genome copy number abnormalitywere common to a pN(+) case as comparedwith a pN(-) case, and tobefound and listed the meta(we) stasis connection gene candidates who were present in theatrical company rank. Furthermore, we inspected the mRNA manifestation situation of the candidate gene by quantitative Real-time PCR.
[Results] In the region that we were common to a pN(+) case, and a genomic copy number increased, 16 regions, region that decreased were 32 regions. We listed 75 existing candidate genes on these regions. A ZDHHC14 gene did locus in the one 6q25.3 region of the region that a copy number specifically increased for a pN(+) case. As a result of having assayed the mRNA expression level of the gene, high-frequency manifestation sthenia was found (40% of 8/20 cases). Furthermore, the mRNA manifestation sthenia was significantly found in a pN(+) case (P=0.019).
[Conclusions] These results suggested the possibility that ZDHHC14 was a new metastasis of the oral squamous cell carcinoma-related gene. As for this study, information as we exceeded imagination with the conducted genomic analysis much more was obtained till now, and the results were given as a new search method surely and this gave a presentation by foreign countries thesis and gave large result.
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[Journal Article] Clinical study of multiple primary and double cancers including oral squamous cell carcinoma
Author(s)
Yamamoto N., Okubo T., Sakuma T., Sugahara K., Yamamoto M., Muramatsu K., Watanabe A., Yakushiji T., Nomura T., Takano N., Shibahara T
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Journal Title
Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology
Volume: (in press)
Peer Reviewed
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[Journal Article] Aneurysmal bone cyst in mandible : Report of 2 cases and review of literature
Author(s)
Sakuma, T., Yamamoto, N., Onda, T., Sugahara, K., Yamamoto, M., Muramatsu, K., Watanabe, A., Kamio, T., Sakamoto, J., Sano, T., Matsuzaka, K., Takano, N., Shibahara, T
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Journal Title
Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology
Volume: (in press)
Peer Reviewed
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[Presentation] Proteomics based identification of differentially expressed proteins in human oral squamous cell carcinoma2011
Author(s)
Onda, T., Sakuma, T., Bessho, H., Yakushiji, T., Yamamoto, N., Nomura, T., Takano, N., Shibahara, T
Organizer
第89回国際口腔外科学会
Place of Presentation
サンディエゴ(チリ)
Year and Date
2011-10-26
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[Presentation] Oral squamous cell carcinoma and cultural risk in Japan2011
Author(s)
Nomura, T., Kawachi, H., Sugahara, K., Ikeda, C., Yakushiji, T., Yamamoto, N., Takano, N., Shibahara, T
Organizer
第89回IADR学会総会
Place of Presentation
サンディエゴ(アメリカ)
Year and Date
2011-03-17
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[Presentation] Verification of gene polymorphism in oral squamous cell carcinoma2010
Author(s)
Kawachi, H., Sugahara, K., Ikeda, C., Yakushiji, T., Yamamoto, N., Nomura, T., Takano, N., Shibahara, T., Katakura, A
Organizer
第9回アジア口腔顎顔面外科学会総会
Place of Presentation
クアラルンプール(マレーシア)
Year and Date
2010-10-26
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[Presentation] Over expression of zinc finger, DHHC-type containing14gene located on chromosome 6q25.3 in human tongue squamous cellcarcinoma2010
Author(s)
Onda, T., Yamamoto, N., Uchiyama, T., Takano, N., Shibahara, T
Organizer
第9回アジア口腔顎顔面外科学会総会
Place of Presentation
クアラルンプール(マレーシア)
Year and Date
2010-10-26
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