2012 Fiscal Year Final Research Report
Implications for novel drug development in bone metabolism: Beyond the dentin extracellular matrix.
| Project/Area Number |
22792040
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthodontic/Pediatric dentistry
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
HARUYAMA Naoto 東京医科歯科大学, 歯と骨のGCOE拠点, GCOE拠点形成特任教員 (70359529)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 細胞外マトリクス / 象牙質シアロリン酸化タンパク / 骨代謝 / 間葉系幹細胞 / 歯学 |
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| Research Abstract |
In this study, we examined the effects of dentin sialophosphoprotein (DSPP), one of the major extracellular matrix proteins in dentin, on bone turnover in vivo and in vitro. According to micro CT analysis, DSPP knock out (KO) mouse femora showed significantly decreased bone mineral density as compared to those in DSPP wild type (WT) mice. However, calvarial organ culture analysis revealed that the decreased osteoclastogenesis in DSPP KO as compared to WT calvaria. Using bone marrow stromal cells (BMSCs) isolated from both WT and KO mice, we found that under the osteogenic media,ALP activity and in vitro mineralization were higher in BMSCs derived from WT mice as compared to those from KO mice. On the other hand, the KO derived BMSCs showed increased differentiation into adipocytes as compared to those from WT mice. Thus, the DSPP may be a positive regulator for the bone mineral density, promoting the bone formation but not suppressing the bone resorption.
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