Long term cryopreservation of bone tissue for clinical use

2011 Fiscal Year Final Research Report

• Project/Area Number: 22792054
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Hiroshima University
• Principal Investigator: KAKU Masato 広島大学, 大学院・医歯薬学総合研究科, 助教 (10325194)
• Project Period (FY): 2010 – 2011
• Keywords: 再生医療 / 骨組織 / 凍結保存 / 磁場 / プログラムフリーザ

Research Abstract

A plunging temperature of-30℃, a hold-time at-5℃for 15 minutes and a 0. 1 mT of a magnetic field led to both the greatest survival and growth rate. Moreover, there was no significant difference in ALP, OPN and BSP mRNA expression and protein concentration between cryopreserved and control groups. New bone formation and bone bridge were detected in 8, 12 and 24-week control groups. In 4-and 8-week cryopreserved and dried groups, there was no new bone formation around the intact bones. New bone formation was observed in 12-week cryopreserved group and the transplanted bone was connected to the intact bone in 24-week cryopreserved group. In 12 and 24 weeks, a large amount of bone resorption in the transplanted area was observed in the dried group. These results suggest that bone tissue cryopreservation can be successfully used for bone grafting which may be a novel option for cranioplasty.

Research Products

• [Journal Article] Effects of cryopreservation with a newly-developedmagnetic field programmed freezer onperiodontal ligament cells and pulp tissues (2011)
  • Author(s): Abedini, S., Kaku, M., Kawata T., Koseki H., Kojima S., Sumi, H., Motokawa M., FujitaT., Ohtani J., Ohwada N., Tanne K
  • Journal Title: Cryobiology Volume: 62 Pages: 181-187
  • Peer Reviewed
• [Journal Article] Cryopreservation of periodontal ligament cells with magnetic field for tooth banking (2010)
  • Author(s): Kaku, M., Kamda H., Kawata T., Koseki H., Abedini S., Kojima S., Motokawa M., Fujita T., Ohtani J., Tsuka N., Matsuda Y., Sunagawa H., Hernandes RA., Ohwada N., Tanne K
  • Journal Title: Cryobiology Volume: 61 Pages: 73-78
• [Presentation] 磁場を利用したラット骨髄由来間葉系幹細胞の長期凍結保存 (2011)
  • Author(s): 小島俊逸, 加来真人, 河田俊嗣, 本川雅英, 藤田正, 大谷淳二, 小跡弘幸, Sara Abedini, 角明美, 丹根一夫
  • Organizer: 第70回日本矯正歯科学会
  • Place of Presentation: 名古屋市
  • Year and Date: 20111017-20
• [Presentation] 磁場を利用した骨組織の長期凍結保存法 (2010)
  • Author(s): 小跡弘幸、加来真人、河田俊嗣、本川雅英、藤田正、大谷淳二、SaraABEDINI、小島俊逸、丹根一夫
  • Organizer: 第69回日本矯正歯科学会
  • Place of Presentation: 横浜市
  • Year and Date: 20100927-29
• [Presentation] 磁場を利用したラット骨髄由来間葉系幹細胞の長期凍結保存法 (2010)
  • Author(s): 小島俊逸、加来真人、河田俊嗣、本川雅英、藤田正、大谷淳二、小跡弘幸、Sara ABEDINI、、丹根一夫
  • Organizer: 第69回日本矯正歯科学会
  • Place of Presentation: 横浜市
  • Year and Date: 20100927-29
• [Remarks]
  • URL: http://teethbank.jp/http://teethbank.jp/