Characterization of Periodontitis Vaccine, Dendritic Cells from Mouse Induced Pluripotent Stem Cells

2011 Fiscal Year Final Research Report

• Project/Area Number: 22792117
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Social dentistry
• Research Institution: Osaka University
• Principal Investigator: SEKINE Shinichi 大阪大学, 大学院・歯学研究科, 助教 (70506344)
• Project Period (FY): 2010 – 2011
• Keywords: 樹状細胞 / ワクチン / iPS細胞 / 歯周病

Research Abstract

We have succeeded to generate dendritic cells(DCs) from mouse induced pluripotent stem(iPS) cells according to reported procedures(Senju et al, Stem cells. 27 : 1021, 2009). To assess the whether iPS-DCs possess Ag-presenting Cell(APC) function, OVA-specific naive CD4^+ cells from OT-II Tg mice were purified by FACSAria^<TM>. The iPS cell-derived DCs(iPS-DCs) were suspended in complete RPMI 1640 and incubated with or without OVA for 24hr. Complete RPMI containing OVA was replaced with OVA-specific CD4^+ T cells for 3 days. CD4^+ T cells co-cultured with iPS-DCs showed higher proliferative responses when compared with control. When we examined adjuvanticity of iPS-DCs, significant levels of mucosal IgA Ab responses were also induced. These results demonstrate that iPS-DCs offer an attractive possibility for an effective mucosal vaccine.

Research Products

• [Journal Article] Notch-ligand expression by NALT dendritic cells regulates mucosal Th1-and Th2-type responses (2012)
  • Author(s): Fukuyama Y, Tokuhara D, Sekine S, Kataoka K, Markham JD, Irwin AR, Moon GH, Tokuhara Y, Fujihashi K, Davydova J, Yamamoto M, Gilbert RS, Fujihashi K
  • Journal Title: Biochem Biophys Res Commun Volume: 418(1) Pages: 6-11
  • Peer Reviewed
• [Journal Article] A nobel combined adjuvant for nasal delivary elicits mucosal immunity to influenza in aging (2012)
  • Author(s): Asanuma H, Zamri NB, Sekine S Fukuyama Y, Tokuhara D, Gilbert RS, Fukuiwa T, Fujihashi K, Sata T, Tashiro M, Fujihashi K
  • Journal Title: Vaccine Volume: 30(4) Pages: 803-12
  • Peer Reviewed
• [Journal Article] Functional transforming growth factor-βreceptor type II expression by CD4^+ T cells in Peyer' s patches is essential for oral tolerance induction (2011)
  • Author(s): Gilbert RS, Kobayashi R, Sekine S, Fujihashi K
  • Journal Title: PLoS One Volume: 6(11) Pages: e27501
  • Peer Reviewed
• [Journal Article] Oral-nasopharyngeal dendritic cells mediate T cell-independent IgA class switching on B-1 B cells (2011)
  • Author(s): Kataoaka K, Fujihashi K, Terao T, Gilbert RS, Sekine S, Kobayashi R, Fukuyama Y, Kawabata S, Fujihashi K
  • Journal Title: PLoS One Volume: 6(9) Pages: e25396
  • Peer Reviewed
• [Journal Article] The nasal dendritic cell-targeting Flt3 ligand as a safe adjuvant elicits effective protection against fatal pneumococcal pneumonia (2011)
  • Author(s): Kataoaka K, Fujihashi K, Oma K, Fukuyama Y, Hollingshead SK, Sekine S, Kawabata S, Ito HO, Briles DE, Oishi K
  • Journal Title: Infect Immun Volume: 79(7) Pages: 2819-28
  • Peer Reviewed
• [Presentation] 歯肉由来iPS細胞を利用した樹状細胞の免疫誘導能解析 (2011)
  • Author(s): 関根伸一, 岩崎未央, 永田英樹, 天野敦雄
  • Organizer: 22^<th>近畿・中国・四国口腔衛生学会総会
  • Place of Presentation: 徳島
  • Year and Date: 2011-10-01
• [Presentation] Effectiveness of pre-administered nasal adjuvants for the induction of influenza-specific IgA antibody responses in the upper respiratory tract (2011)
  • Author(s): Asanuma H, Sekine S, Fukuyama Y, Ainai A, Hsegawa H, Tashiro M, Fujihashi K
  • Organizer: 15^<th> ICMI
  • Place of Presentation: Paris(France)
  • Year and Date: 2011-07-08
• [Presentation] Nasal dendritic cell targeting Flt3 ligand as a safe adjuvant elicits effective protection against fatal pneumococcal pneumonia (2011)
  • Author(s): Kataoka K, Oma K, Fukuyama Y, Hollingshead S, Sekine S, Kawabata S, Briles D, Oishi K, Fujihashi K
  • Organizer: 15^<th> ICMI
  • Place of Presentation: Paris(France)
  • Year and Date: 2011-07-08
• [Presentation] Kinetic Analysis of the Development of Effector-and Memory-type CD4^+ T Cells in the Intestinal Mucosa (2011)
  • Author(s): Kobayashi R, Sekine S, Fujihashi K, Gilbert RS, Fukuyama Y, Yamamoto M, Fujihashi K
  • Organizer: 15^<th> ICMI
  • Place of Presentation: Paris(France)
  • Year and Date: 2011-07-07
• [Presentation] CCR5 and CCR6 Play Central Roles in Adenovirus Expressing Flt3 Ligand-induced NALT Dendritic Cell Migration and S-IgA Ab Responses in Mucosal Effector Tissues (2011)
  • Author(s): Fukuyama Y, Sekine S, Kobayashi R, Gilbert RS, Fujihashi K
  • Organizer: 15^<th> ICMI
  • Place of Presentation: Paris(France)
  • Year and Date: 2011-07-06
• [Presentation] F. nucleatum表層タンパク質FomAと唾液高チロシンタンパク質スタセリンペプチドとの相互作用 (2010)
  • Author(s): 片岡宏介, 中垣晴男, 関根伸一, 福井誠, 伊藤博夫, 雫石聰
  • Organizer: 53^<th>春期日本歯周病学会
  • Place of Presentation: 盛岡
  • Year and Date: 20100514-05
• [Presentation] 佐用町台風9号災害における住民の口腔保健調査 (2010)
  • Author(s): 関根伸一, 片岡宏介, 東江正裕, 橋野恵衣, 岩崎未央, 田中宗雄, 新庄文明
  • Organizer: 59^<th>日本口腔衛生学会
  • Place of Presentation: 新潟
  • Year and Date: 2010-10-08
• [Presentation] ウラジロガシ抽出物配合チューイングガムによる口臭の即効性消臭効果:クロスオーバー二重盲検査試験 (2010)
  • Author(s): 東江正裕, 関根伸一, 橋野恵衣, 岩崎未央, 田中宗雄
  • Organizer: 59^<th>日本口腔衛生学会
  • Place of Presentation: 新潟
  • Year and Date: 2010-10-07
• [Presentation] 遺伝子改変植物による歯周病原菌定着阻害を有するヒト唾液タンパク質スタセリンペプチドの発現 (2010)
  • Author(s): 片岡宏介, 関根伸一, Tselmeg B, Sapta M, 福井誠, 金川裕子, 伊藤博夫
  • Organizer: 59^<th>日本口腔衛生学
  • Place of Presentation: 新潟
  • Year and Date: 2010-10-07
• [Presentation] Flt3 ligand cDNA及びCpG ODNは粘膜アジュバントとしてP. gingivalis抗原特異的抗体反応を誘導する (2010)
  • Author(s): 関根伸一, 片岡宏介, 橋野恵衣, 岩崎未央, 田中宗雄, 永田英樹
  • Organizer: 53^<th>秋期日本歯周病学会
  • Place of Presentation: 高松
  • Year and Date: 2010-09-09
• [Presentation] 粘膜アジュバントCpGオリゴヌクレオチドによるP. gingivalis線毛抗原特異的抗体反応の誘導 (2010)
  • Author(s): 片岡宏介, 関根伸一, 福井誠, 横山正明, 伊藤博夫
  • Organizer: 53^<th>秋期日本歯周病学会
  • Place of Presentation: 高松
  • Year and Date: 2010-09-09
• [Presentation] Characterization of mucosal immunosenescence in nasopharyngeal-associate lymphoreticular tissue (2010)
  • Author(s): Sekine S, Kobayashi R, Sasaki M, Fukuyama Y, Asanuma H, Gilbert RS, Fujihashi K, Kataoka K, Fujihashi K
  • Organizer: 14^<th> ICI
  • Place of Presentation: Kobe(Japan)
  • Year and Date: 2010-08-26
• [Presentation] Both mucosal and systemic IgA responses are enhanced in orally immunized PIR-B-deficient mice (2010)
  • Author(s): Kobayashi R, Kubagawa H, Takai T, Sekine S, Fujihashi K, Gilbert RS, Fukuyama Y, Fujihashi K
  • Organizer: 14^<th> ICI
  • Place of Presentation: Kobe(Japan)
  • Year and Date: 2010-08-26
• [Presentation] Nasal influenza vaccines containing pFL and CpG ODN as mucosal adjuvant elicit crossreactive functional S-IgA antibody (2010)
  • Author(s): Asanuma H, Sekine S, Suzuki Y, Sata T, Tashiro M, Fujihashi K
  • Organizer: 14^<th> ICI
  • Place of Presentation: Kobe(Japan)
  • Year and Date: 2010-08-25
• [Presentation] Nasal cholera toxin activated mucosal dendritic cells, but not CD4^+ T cells elicit TI-IgA class switching recombination by B-1 B cells (2010)
  • Author(s): Kataoka K, Fujihashi K, Kobayashi R, Gilbert RS, Sekine S, Fukui M, Fukuyama Y, Kawabata S, Ito HO, Fujihashi K
  • Organizer: 14^<th> ICI
  • Place of Presentation: Kobe(Japan)
  • Year and Date: 2010-08-24
• [Remarks]
  • URL: http://web.dent.osaka-u.ac.jp/~prevent/index.html