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2011 Fiscal Year Final Research Report

Analysis of the molecular mechanism of sperm head formation using gene-manipulated animals

Research Project

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Project/Area Number 22800041
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Laboratory animal science
Research InstitutionOsaka University

Principal Investigator

FUJIHARA Yoshitaka  大阪大学, 微生物病研究所, 特任研究員 (70578848)

Project Period (FY) 2010 – 2011
Keywords遺伝子組換えマウス / 精子形成 / 巨大円形精子症 / SPACA1タンパク質
Research Abstract

SPACA1 is a membrane protein that localizes in the equatorial segment of spermatozoa in mammals and is reported to function in sperm. egg fusion. We produced a Spaca1 gene-disrupted mouse line and found that the male mice were infertile. However, the cause of this sterility turned out to be abnormal shaping of the sperm head reminiscent of globozoospermia in humans. Disruption of Spaca1 led to disappearance of the nuclear plate, a dense lining structure of the nuclear envelope facing the inner acrosomal membrane. This coincided with the failure of acrosomal expansion during spermiogenesis and resulted in the degeneration and disappearance of the acrosome in mature spermatozoa. Thus, one of the mechanisms leading to globozoospermia has been clarified.

  • Research Products

    (9 results)

All 2011 2010

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (5 results) Book (2 results)

  • [Journal Article] Sperm equatorial segment protein 1, SPESP1, is required for fully fertile sperm in mous2010

    • Author(s)
      Fujihara Y., Murakami M., Inoue N., Satouh Y., Kaseda K., Ikawa M., Okabe M.
    • Journal Title

      Journal of Cell Science

      Volume: 123号 Pages: 1531-1536

    • DOI

      doi:10.1242/jcs.067363

    • Peer Reviewed
  • [Journal Article] The X-linked imprinted gene family Fthl17 shows predominantly female expression following the two-cell stage in mouse embryos2010

    • Author(s)
      Kobayashi S., Fujihara Y., Mise N., Kaseda K., Abe K., Ishino F., Okabe M.
    • Journal Title

      Nucleic Acids Research

      Volume: 38号 Pages: 3672-81

    • DOI

      doi:10.1093/nar/gkq113

    • Peer Reviewed
  • [Presentation] 精巣内生殖細胞特異的な発現を示すTex101ノックアウトマウスの作製と機能解析2011

    • Author(s)
      藤原祥高、徳弘圭造、室悠子、荒木慶彦、伊川正人、岡部勝
    • Organizer
      第34回日本分生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2011-12-13
  • [Presentation] 精子形成におけるIZUMO1およびSPACA1タンパク質の局在変化と機能解析2011

    • Author(s)
      藤原祥高、佐藤裕公、井上直和、磯谷綾子、伊川正人、岡部勝
    • Organizer
      第58回日本実験動物学会総会
    • Place of Presentation
      東京
    • Year and Date
      2011-05-25
  • [Presentation] Spaca1, sperm acrosome associated 1, is required for acrosome formation in mice2010

    • Author(s)
      藤原祥高、佐藤裕公、井上直和、磯谷綾子、伊川正人、岡部勝
    • Organizer
      BMB2010(第33回日本分生物学会年会、第83回日本生化学会大会)
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-07
  • [Presentation] Sperm equatorial segment protein 1, SPESP1, is required for fully fertile sperm in mouse2010

    • Author(s)
      Y. Fujihara, M. Murakami, N. Inoue, Y. Satouh, K. Kaseda, M. Ikawa, M. Okabe
    • Organizer
      International Symposium for Immunology of Reproduction
    • Place of Presentation
      Osaka, Japan
    • Year and Date
      2010-08-28
  • [Presentation] Spaca1, Sperm acrosome associated 1, is required for sperm-head shaping2010

    • Author(s)
      Y. Fujihara, Y. Satouh, N. Inoue, A. Isotani, M. Ikawa, M. Okabe
    • Organizer
      11th International Symposium on Spermatology
    • Place of Presentation
      Okinawa, Japan
    • Year and Date
      2010-07-24
  • [Book] 「疾患モデルマウス:臓器系:生殖細胞」、『疾患モデルマウス表現型解析指南』2011

    • Author(s)
      藤原祥高、伊川正人
    • Total Pages
      213-220
    • Publisher
      株式会社中山書店
  • [Book] ノックアウトマウスプロジェクト」、『モデル動物利用マニュアル<生物機能モデルと新しいリソース・リサーチツール>』2011

    • Author(s)
      森岡裕香、藤原祥高、伊川正人
    • Total Pages
      459-468
    • Publisher
      株式会社エル・アイ・シー

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Published: 2013-07-31  

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