2011 Fiscal Year Final Research Report
Development of muliticolor fluorescent assay based on nucleic acid-binding ligands
| Project/Area Number |
22850001
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Analytical chemistry
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| Research Institution | Tohoku University |
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Principal Investigator |
SATO Yusuke 東北大学, 大学院・理学研究科, 助教 (90583039)
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| Project Period (FY) |
2010 – 2011
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| Keywords | AP site / 蛍光性リガンド / シアニン誘導体 / マルチカラー蛍光解析 / 遺伝子解析 |
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| Research Abstract |
A series of conjugates of abasic site-binding ligands with fluorescent signaling intercalators have been synthesized with a view toward gene analysis, especially single nucleotide polymorphisms (SNPs). With focusing on the variability of the emission wavelength of DNA-binding cyanine dyes by modification of their structures, blue, green, red fluorescence signaling conjugates have been developed for the selective detection of pyrimidine nucleobase over purine nucleobases.
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