2011 Fiscal Year Final Research Report
Characterization of foulant in membrane bioreactors for elucidation of fouling mechanisms
| Project/Area Number |
22860001
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Civil and environmental engineering
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| Research Institution | Hokkaido University |
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Principal Investigator |
MIYOSHI Taro 北海道大学, 環境ナノ・バイオ工学研究センター, 博士研究員 (80587791)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 膜分離活性汚泥法 / 膜閉塞 / 膜閉塞成分特性解析 |
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| Research Abstract |
In this study, detailed characterization of polysaccharides and proteins that caused membrane fouling in membrane bioreactors(MBRs) was carried out. A mild acid hydrolysis of polysaccharides eluted from a lectin column followed by MALDI-TOF/MS analysis allowed us to have deeper insights into structures of polysaccharides that have high fouling potentials. The proteins extracted from fouled membranes were successfully concentrated by the combination of a crude concentration with ultra-filtration(UF) membrane and trichloroacetic acid(TCA) precipitation. The concentrated proteins were separated well by 2D-PAGE. Analyzing selected 2D-PAGE spots by N-terminal amino acid sequencing analysis led to the identification of two well-characterized outer membrane proteins originating from Pseudomonas genus
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