2011 Fiscal Year Final Research Report
Structural basis for the indirect aminoacyl-tRNA synthesis pathway from H. pyroli
| Project/Area Number |
22870001
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Structural biochemistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
NAKAMURA Akiyoshi 北海道大学, 大学院・先端生命科学研究院, 博士研究員 (10583891)
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| Project Period (FY) |
2010 – 2011
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| Keywords | アミノアシルtRNA合成酵素 / tRNA / X線結晶構造 |
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| Research Abstract |
We have been focusing on the structural and functional analysis of GatCAB, which has an important role in an indirect pathway of aminoacyl-tRNA synthesis. We have determined the crystal structure of an archaeal GatCAB at 2. 5 A resolution. The structural comparison of the archaeal GatCAB and the bacterial GatCAB suggested that a 3_<10> helix in the bacterial GatCAB is crucial for recognition the U1-A72 pair in tRNA^<Gln>, and the absence of the helix in the archaeal GatCAB enables the enzyme recognize aa-tRNA with G1-C72 base-pairs.
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