2011 Fiscal Year Final Research Report

Analysis of thioredoxin mediated redox-regulation of nitrogenase activity

Research Project

Project/Area Number	22870009
Research Category	Grant-in-Aid for Research Activity Start-up
Allocation Type	Single-year Grants
Research Field	Plant molecular biology/Plant physiology
Research Institution	Tokyo Institute of Technology
Principal Investigator	NOMATA Jiro 東京工業大学, 資源化学研究所, 助教 (40583216)
Project Period (FY)	2010 – 2011
Keywords	レドックス / 酸素感受性 / シアノバクテリア
Research Abstract	Fe-protein, a reductant component of nitrogenase, was expressed in E. coli and successfully purified under anaerobic condition. 2. Thioredoxin A was expressed in E. coli and successfully purified in active form.