2011 Fiscal Year Final Research Report
Analysis on the higher-order chromatin structure in the interphase nucleus using super-resolution fluorescence microscopy
| Project/Area Number |
22870042
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Structural biochemistry
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| Research Institution | National Institute of Information and Communications Technology |
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Principal Investigator |
MATSUDA Atsushi 独立行政法人情報通信研究機構, 未来ICT研究所・バイオICT研究室, 専攻研究員 (20585723)
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| Project Period (FY) |
2010 – 2011
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| Keywords | クロマチン / 分裂酵母 / 超分解能蛍光顕微鏡 |
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| Research Abstract |
I developed a method for live observation using super-resolution microscopy, 3DSIM, and applied on live fission yeast. The interphase chromatin in live fission yeast formed fibrous structures with a diameter of 100-150 nm. Chromatin in fixed fission yeast nuclei was stained with antibodies against modified histones and observed with 3DSIM, then the level of overlap with the DNA mass was quantitatively analyzed. The results showed that regions associated with ongoing transcription were de-condensed and extended from fibrous structures.
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[Journal Article] Fast live simultaneous multiwavelength four-dimensional optical microscopy2010
Author(s)
Carlton PM, Boulanger J, Kervrann C, Sibarita JB, Salamero J, Gordon-Messer S, Bressan D, Haber JE, Haase S, Shao L, Winoto L, Matsuda A, Kner P, Uzawa S, Gustafsson M, Kam Z, Agard DA, Sedat JW
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Journal Title
Proc Natl Acad Sci USA
Volume: 107(37)
Pages: 16016-16022
DOI
Peer Reviewed
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[Journal Article] Condensed mitotic chromosome structure at nanometer resolution using PALM and EGFP-Histones2010
Author(s)
Matsuda, A., Shao, L., Boulanger, J., Kervrann, C., Carlton, P. M., Kner, P., Agard, D., and Sedat, J. W.
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Journal Title
PLoS one
Volume: 5
Pages: 12768
DOI
Peer Reviewed
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