Structure and function of a novel signal transducer protein that employs heme-PAS domain as a sensor

2011 Fiscal Year Final Research Report

• Project/Area Number: 22870043
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Structural biochemistry
• Research Institution: 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設) (2011); National Institutes of Natural Sciences Okazaki Research Facilities (2010)
• Principal Investigator: SAWAI Hitomi 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 特任助教 (50584851)
• Project Period (FY): 2010 – 2011
• Keywords: ヘムタンパク質 / センサータンパク質 / シグナル伝達タンパク質 / 走化性制御 / ヘム含有PASドメイン / 共鳴ラマン分光法 / X線結晶構造解析

Research Abstract

I studied the structure and function of Aer2, a novel signal transducer protein for bacterial aerotaxis control. Spectroscopy and site-directed mutagenesis revealed that Aer2 was a new type of oxygen sensor protein employing a heme as a sensor. To elucidate the molecular mechanisms of oxygen sensing and signal transduction, I characterized Aer2 by X-ray crystallography and resonance Raman spectroscopy.

Research Products

• [Journal Article] Structural basis for oxygen sensing and signal transduction of the heme-based sensor protein Aer2 from Pseudomonas aeruginose (2012)
  • Author(s): Hitomi Sawai, Hiroshi Sugimoto, Yoshitsugu Shiro, Haruto Ishikawa, Yasuhisa Mizutani, Shigetoshi Aono
  • Journal Title: Chemical Communications Volume: 48 Pages: 6523-6525
  • Peer Reviewed
• [Presentation] Structural characterization of Aer2 that employs a heme as an oxygen sensor for aerotaxis control (2012)
  • Author(s): Hitomi Sawai, Hiroshi Sugimoto, Haruto Ishikawa, Yasuhisa Mizutani, Yoshitsugu Shiro, Shigetoshi Aono
  • Organizer: 4^<th> Japan-Korea Seminars on Biomolecular Sciences : Experiments and Simulations
  • Place of Presentation: Todaiji Cultural Center(Japan)
  • Year and Date: 20120109-11
• [Presentation] Structural characterization of Aer2 that adopts a heme-containing PAS domain as an oxygen sensor for aerotaxis control (2011)
  • Author(s): Hitomi Sawai, Hiroshi Sugimoto, Haruto Ishikawa, Yoshitsugu Shiro, Yasuhisa Mizutani, Shigetoshi Aono
  • Organizer: 15^<th> International Conference on Biological Inorganic Chemistry(ICBIC15)
  • Place of Presentation: University of British Colombia(Canada)
  • Year and Date: 20110807-12
• [Presentation] ヘム含有PASドメインを有する新規な走化性制御蛋白質Aer2の酸素感知機構 (2010)
  • Author(s): 澤井仁美、石川春人、水谷泰久、青野重利
  • Organizer: 第4回バイオ関連化学合同シンポジウム
  • Place of Presentation: 大阪大学(大阪府)
  • Year and Date: 20100924-26
• [Presentation] ガス分子により駆動される新規なセンサータンパク質の機能発現機構 (2010)
  • Author(s): 澤井仁美、青野重利
  • Organizer: 平成22年度特定領域「細胞感覚」夏の班会議
  • Place of Presentation: 札幌市教育文化会館(北海道)
  • Year and Date: 20100705-06