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2011 Fiscal Year Final Research Report

Structure and function of a novel signal transducer protein that employs heme-PAS domain as a sensor

Research Project

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Project/Area Number 22870043
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Structural biochemistry
Research Institution大学共同利用機関法人自然科学研究機構(岡崎共通研究施設) (2011)
National Institutes of Natural Sciences Okazaki Research Facilities (2010)

Principal Investigator

SAWAI Hitomi  大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 特任助教 (50584851)

Project Period (FY) 2010 – 2011
Keywordsヘムタンパク質 / センサータンパク質 / シグナル伝達タンパク質 / 走化性制御 / ヘム含有PASドメイン / 共鳴ラマン分光法 / X線結晶構造解析
Research Abstract

I studied the structure and function of Aer2, a novel signal transducer protein for bacterial aerotaxis control. Spectroscopy and site-directed mutagenesis revealed that Aer2 was a new type of oxygen sensor protein employing a heme as a sensor. To elucidate the molecular mechanisms of oxygen sensing and signal transduction, I characterized Aer2 by X-ray crystallography and resonance Raman spectroscopy.

  • Research Products

    (5 results)

All 2012 2011 2010

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (4 results)

  • [Journal Article] Structural basis for oxygen sensing and signal transduction of the heme-based sensor protein Aer2 from Pseudomonas aeruginose2012

    • Author(s)
      Hitomi Sawai, Hiroshi Sugimoto, Yoshitsugu Shiro, Haruto Ishikawa, Yasuhisa Mizutani, Shigetoshi Aono
    • Journal Title

      Chemical Communications

      Volume: 48 Pages: 6523-6525

    • Peer Reviewed
  • [Presentation] Structural characterization of Aer2 that employs a heme as an oxygen sensor for aerotaxis control2012

    • Author(s)
      Hitomi Sawai, Hiroshi Sugimoto, Haruto Ishikawa, Yasuhisa Mizutani, Yoshitsugu Shiro, Shigetoshi Aono
    • Organizer
      4^<th> Japan-Korea Seminars on Biomolecular Sciences : Experiments and Simulations
    • Place of Presentation
      Todaiji Cultural Center(Japan)
    • Year and Date
      20120109-11
  • [Presentation] Structural characterization of Aer2 that adopts a heme-containing PAS domain as an oxygen sensor for aerotaxis control2011

    • Author(s)
      Hitomi Sawai, Hiroshi Sugimoto, Haruto Ishikawa, Yoshitsugu Shiro, Yasuhisa Mizutani, Shigetoshi Aono
    • Organizer
      15^<th> International Conference on Biological Inorganic Chemistry(ICBIC15)
    • Place of Presentation
      University of British Colombia(Canada)
    • Year and Date
      20110807-12
  • [Presentation] ヘム含有PASドメインを有する新規な走化性制御蛋白質Aer2の酸素感知機構2010

    • Author(s)
      澤井仁美、石川春人、水谷泰久、青野重利
    • Organizer
      第4回バイオ関連化学合同シンポジウム
    • Place of Presentation
      大阪大学(大阪府)
    • Year and Date
      20100924-26
  • [Presentation] ガス分子により駆動される新規なセンサータンパク質の機能発現機構2010

    • Author(s)
      澤井仁美、青野重利
    • Organizer
      平成22年度特定領域「細胞感覚」夏の班会議
    • Place of Presentation
      札幌市教育文化会館(北海道)
    • Year and Date
      20100705-06

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Published: 2013-07-31  

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