2011 Fiscal Year Final Research Report

DNA barcoding to detect putative hybrid species in bamboos

Research Project

Project/Area Number	22880010
Research Category	Grant-in-Aid for Research Activity Start-up
Allocation Type	Single-year Grants
Research Field	Forest science
Research Institution	The University of Tokyo
Principal Investigator	HISAMOTO Yoko 東京大学, 大学院・農学生命科学研究科, 助教 (60586014)
Project Period (FY)	2010 – 2011
Keywords	ササ類 / 推定雑種分類群 / 核遺伝子マーカー / 葉緑体遺伝子マーカー / 花成促進遺伝子FLOWERING LOCUS T(FT)
Research Abstract	Japanese dwarf bamboos(Sasa-group) develop hybrids easily, and the identification on the basis of the external morphologic traits of vegetative organs is difficult. The aim of this study was to investigate the usefulness of chloroplast and nuclear genes as DNA markers for differentiation between Sasa-group hybrid taxa. Thirty-seven and sixteen samples were collected in the University of Tokyo Chichibu Forest and the University of Tokyo Hokkaido Forest, respectively. Intergeneric and interspecific hybrids were identified on the basis of external morphologic traits. At the result of molecular analysis, one of the flowering genes, FLOWERING LOCUS T(FT) may be a useful DNA marker to detect hybrids.