The mechanisms of DNA demethylation by APOBEC2 deaminase during myogenic differentiation

2011 Fiscal Year Final Research Report

• Project/Area Number: 22880025
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Zootechnical science/Grassland science
• Research Institution: Kyushu University
• Principal Investigator: SATO Yusuke 九州大学, 農学研究院, 特任助教 (50589520)
• Project Period (FY): 2010 – 2011
• Keywords: 骨格筋 / APOBEC2 / 筋分化 / 筋再生 / DNA脱メチル化

Research Abstract

The aim of the present research was to understand the mechanisms of skeletal muscle growth and hypertrophy. We hypothesized that the myogenic differentiation was regulated by DNA demethylation via deamination by APOBEC2 enzyme. As a result, we found that lack of APOBEC2 promoted the myogenic differentiation. APOBEC2 might negatively regulate muscle differentiation.

Research Products

• [Journal Article] Growth Factor Regulation of Neural Chemorepellent SemasA Expression in Satellite Cell Cultures (2011)
  • Author(s): Do MK Q, Sateo Y, Shimizu N, Suzuki T, ShenaaIII, Mizunoya W, Nakamura M, Ikeuchi Y, Anderson IE, and Tatsumi R
  • Journal Title: American Journal of Physiology-Cell Physiology Volume: 301 Pages: C1270-C1279
  • Peer Reviewed
• [Presentation] semaphorin 3Aは筋幹細胞の初期分化を制御する (2012)
  • Author(s): 鈴木貴弘,尾嶋孝一,佐藤祐介,中村真子,水野谷航,辰巳隆一,池内義秀
  • Organizer: 日本畜産学会第115回大会
  • Place of Presentation: 名古屋大学東山キャンパス
  • Year and Date: 20120327-30
• [Presentation] n-3系不飽和脂肪酸による骨格筋線維タイプの変換 (2012)
  • Author(s): 小宮佑介,水野谷航後藤剛,高橋信之,河田照雄,中村真子,佐藤祐介,辰巳隆一,池内義秀
  • Organizer: 日本畜産学会第115回大会
  • Place of Presentation: 名古屋大学東山キャンパス
  • Year and Date: 20120327-30
• [Presentation] 筋特異的脱アミノ化酵素APOBEC2の筋細胞内局在 (2011)
  • Author(s): 佐藤祐介,大坪秀明,水野谷航,辰巳隆一,池内義秀
  • Organizer: 第114回日本畜産学会大会
  • Place of Presentation: 北里大学十和田キャンパス
  • Year and Date: 20110826-27
• [Presentation] TGF一β2, 3は衛星細胞のSemasA発現調節に関与す (2011)
  • Author(s): ド・マイコイ,清水直美,佐藤祐介,水野谷航中村真子,辰巳隆一,池内義秀
  • Organizer: 第114回日本畜産学会大会
  • Place of Presentation: 北里大学十和田キャンパス
  • Year and Date: 20110826-27
• [Presentation] 筋幹細胞が分泌するsemaphorinsAの生理機能 (2011)
  • Author(s): 鈴木貴弘尾嶋孝一,佐藤祐介,水野谷航中村真子,辰巳隆一,池内義秀
  • Organizer: 第114回日本畜産学会大会
  • Place of Presentation: 北里大学十和田キャンパス
  • Year and Date: 20110826-27
• [Presentation] 筋衛星細胞の活性化における物理刺激受容機構の解明 (2011)
  • Author(s): 原美菜子,田畑久仁子,真鍋宣隆佐藤血,水野谷航,中村真子,辰巳隆一,西村]]三太郎,田畑正志,池内義秀
  • Organizer: 第114回日本畜産学会大会
  • Place of Presentation: 北里大学十和田キャンパス
  • Year and Date: 20110826-27
• [Presentation] 伸展刺激強度の違いが筋衛星細胞の活性化に及ぼす影響 (2011)
  • Author(s): 真鍋宣隆,原美菜子,佐藤祐介,水野谷航,中村真子,辰巳隆一,池内義秀
  • Organizer: 第1144回日本畜産学会大会
  • Place of Presentation: 北里大学十和田キャンパス
  • Year and Date: 20110826-27
• [Presentation] 活性化マクロファージは骨格筋再生に関与する (2011)
  • Author(s): 生野淳一,坂口昇平,佐藤祐介,水野谷航,中村真子,辰巳隆一,池内義秀
  • Organizer: 第114回日本畜産学会大会
  • Place of Presentation: 北里大学十和田キャンパス
  • Year and Date: 20110826-27
• [Presentation] 大豆イソフラボン摂取がラットの骨格筋線維型に及ぼす影響 (2011)
  • Author(s): 下村健太,水野谷航,中村真子,佐藤祐介,辰巳隆一,池内義秀
  • Organizer: 第114回日本畜産学会大会
  • Place of Presentation: 北里大学十和田キャンパス
  • Year and Date: 20110826-27
• [Presentation] on Theme 3 : Tissue Mechanics, Track 3. 6 Muscle Mechanics and Motor Control, the 6th World Congress on Biomechanics 2010, in conjunction with the 14th International Conference on Biomedical Engineering(ICBME) 2010 and the 5th Asian Pacific Conference on (2010)
  • Author(s): Tatsumi R, Do MO, Shimizu N, Sankoda Y, Anderson JE, Sate Y, SuzukiT, Mizunoya W, Ikeuchi Y, and Allen RE
  • Organizer: Possible implication of satellite cells in regenerative motoneuritogenesis : HGF and FGF2 up regulate neural chemorepellent SemasA expression
  • Place of Presentation: Suntec Convention and Exhibition Center, Singapore
  • Year and Date: 20100801-06
• [Presentation] FASEB Summer Research Conference onSkeletal Muscle Satellite & Stem Cells (2010)
  • Author(s): Sate Y, Probst HC, Mizunoya W, Tatsumi R, Rada C, Neuberger MS, and Ikeuchi Y
  • Organizer: Skeletal Muscle Fiber-Type Change, Diminished Body Mass and Myopathy in APOBEC2 KO Mouse
  • Place of Presentation: Carefree Resort, Carefree, AZ, US
  • Year and Date: 20100718-23
• [Presentation] FASEB Summer Research Conference onSkeletal Muscle Satellite & Stem Cells (2010)
  • Author(s): Do MQ, Shimizu N, Sate Y, Suzuki T, Tatsumi R, Mizunoya W, Ikeuchi Y, Anderson JE and Allen RE
  • Organizer: Possible implication of satellite cells in regenerative motoneuritogenesis : Temporal coordination of HGF/FGF2/TGF-b may regulate neural chemorepellent SemasA expression
  • Place of Presentation: Carefree Resort, Carefree, AZ, US
  • Year and Date: 20100718-23
• [Remarks]
  • URL: http://bbsl.agr.kyushu-u.ac.jp/biosci-biotech/chikusan/
• [Remarks]
  • URL: http://agri.mine.utsunomiya-u.ac.jp/hpj/deptj/anij/item/sato.html