Identification and spatiotemporal dynamics of biofilm matrix component proteins in Staphylococcus aureus

2011 Fiscal Year Final Research Report

• Project/Area Number: 22880034
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Applied microbiology
• Research Institution: Jikei University School of Medicine
• Principal Investigator: SUGIMOTO Shinya 東京慈恵会医科大学, 医学部, 助教 (60464393)
• Project Period (FY): 2010 – 2011
• Keywords: バイオフィルム / MRSA / セリンプロテアーゼ / プロテオーム解析 / 分子シャペロン / 細胞骨格タンパク質 / 細胞外マトリックス / 細菌間干渉

Research Abstract

Staphylococcus aureus exhibits a strong capacity to attach to the surface of implanted medical devices and forms multilayered communities of bacteria, called biofilm. S. aureus is a frequent cause of biofilm-associated infections that are a tremendous burden on our healthcare system. Here, we identified and characterized biofilm-matrix proteins which hold cells and contribute to bacterial accumulation in multiple layers. In addition, we found that extracellular serine protease Esp, secreted by a subset of commensal Staphylococcus epidermidis, inhibits nasal colonization of S. aureus via degradation of S. aureus surface proteins and host receptors that are crucial for biofilm formation and host-pathogen interactions. These findings not only provide novel insights into fundamental principles underlying S. aureus biofilm development, but may also indicate a novel direction for therapeutic intervention against biofilm infections.

Research Products

• [Journal Article] Cloning, expression and purification of extracellular serine protease Esp, a biofilm-degrading enzyme, from Staphylococcus epidermidis (2011)
  • Author(s): Shinya Sugimoto, Tadayuki Iwase, Fumiya Sato, Akiko Tajima, Hitomi Shinji, and Yoshimitsu Mizunoe
  • Journal Title: J. Appl. Microbiol Volume: 111 Pages: 1406-1415
  • Peer Reviewed
• [Presentation] Staphylococcus epidermidis Esp degrades novel biofilm matrix proteins in Staphylococcus aureus biofilms (2012)
  • Author(s): Shinya Sugimoto, Takeo Iwamoto, Koji Takada, Fumiya Sato, Tadayuki Iwase, Akiko Tajima, Ippei Hironaka, Ken. ichi Okuda, Hitomi Shinji, and Yoshimitsu Mizunoe
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎
  • Year and Date: 20120327-29
• [Presentation] Cloning, expression and purification of extracellular serine protease Esp, a biofilm-degrading enzyme, from Staphylococcus epidermidis (2012)
  • Author(s): Ken. ichi Okuda, Shinya Sugimoto, Tadayuki Iwase, Fumiya Sato, Akiko Tajima, Hitomi Shinji, and Yoshimitsu Mizunoe
  • Organizer: 2012 Annual Conference of the Association for General and Applied Microbiology
  • Place of Presentation: Tubingen, Germany
  • Year and Date: 20120318-21
• [Presentation] Proteomic survey for Staphylococcus aureus biofilm matrix proteins : extracellular AAA+chaperone ClpB contributes to the biofilm formation (2011)
  • Author(s): Shinya Sugimoto, Takeo Iwamoto, Fumiya Sato, Akiko Tajima, Tadayuki Iwase, Hitomi Shinji, and Yoshimitsu Mizunoe
  • Organizer: 9th International Conference on AAA Proteins
  • Place of Presentation: Kumamoto
  • Year and Date: 20111106-10
• [Presentation] 細胞外分子シャペロンによる黄色ブドウ球菌のバイオフィルム形成機構 (2011)
  • Author(s): 杉本真也, 岩本武夫, 佐藤文哉, 田嶌亜希子, 岩瀬忠行, 進士ひとみ, 水之江義充
  • Organizer: 日本細菌学会関東支部総会
  • Place of Presentation: 東京
  • Year and Date: 20111006-07
• [Presentation] Efficient and economic extracellular production and purification of Staphylococcus epidermidis Esp using a Brevibacillus choshinensis secretion system (2011)
  • Author(s): Shinya Sugimoto, Tadayuki Iwase, Fumiya Sato, Akiko Tajima, Hitomi Shinji, Yukiko Yoshizawa, and Yoshimitsu Mizunoe
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo
  • Year and Date: 20110906-10
• [Remarks]
  • URL: http://square.umin.ac.jp/saikin/index.html