2011 Fiscal Year Final Research Report
Structure analyses of dextran synthases
| Project/Area Number |
22880043
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied biochemistry
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| Research Institution | Hiroshima University |
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Principal Investigator |
HONDOH Hironori 広島大学, 大学院・生物圏科学研究科, 講師 (10368003)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 糖質関連酵素 / 基質特異性 |
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| Research Abstract |
We aimed to reveal the substrate recognition mechanism of isomaltooligosaccharide synthases and hydrolases with their crystal structures. With the crystal structure of DGase, isolamtooligosaccharide hydrolase, it is proposed that the water drain structure at the bottom of the active pocket. We study the effect of the site-directed mutagenesis in this water drain on the hydrolysis activity of this enzyme. The hydrolysis activity was decreased significantly without any difference in its structure.
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