2011 Fiscal Year Final Research Report
Effect of oxidative stress on disulfide bond formation of uric acid efflux transporter BCRP
| Project/Area Number |
22890003
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied pharmacology
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| Research Institution | Hokkaido University |
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Principal Investigator |
OGURA Jiro 北海道大学, 大学院・薬学研究院, 助教 (20580640)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 痛風 / キサンチンオキシダーゼ / 活性酸素 / トランスポータ / ジスルフィド結合 |
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| Research Abstract |
In this study, we investigated that oxidative stress affected BCRP expression, which is a uric acid efflux transporter. It has known that aging-induced oxidative stress is caused by activation of xanthine oxidase. Thus, we used the substrates of xanthine oxidase in this study. In Caco-2 cells, the activation of xanthine oxidase was suppressed the BCRP S-S bond formation, but not in HK-2 cells. These results suggest that the effect of oxidative stress on the efflux of uric acid is different between intestine and kidney.
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