2011 Fiscal Year Final Research Report
Development of highly sensitive methods to analyze signal transduction in a small smooth muscle
| Project/Area Number |
22890011
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
General physiology
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| Research Institution | Asahikawa Medical College |
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Principal Investigator |
TAKEYA Kosuke 旭川医科大学, 医学部, 助教 (20586862)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 平滑筋 / 毛様体筋 / ミオシン / リン酸化定量法 / キナーゼ / ホスファターゼ |
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| Research Abstract |
In order to address the regulatory mechanisms of smooth muscle contraction and relaxation, we aimed to develop a novel highly sensitive method to analyze phosphorylation signals. We are attempting to quantify MYPT1 phosphorylation in bovine ciliary muscle by using phos-tag electrophoresis. High level of myosin phosphorylation was observed in Carbachol-contracted ciliary muscle. Contrary to our expectations, myosin phosphorylation remained at high level even in the resting state. These results suggest that there could be an unknown Ca^<2+>-dependent factor that regulates bovine ciliary muscle contraction rather than myosin phosphorylation.
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