2011 Fiscal Year Final Research Report
The charactarization of herpesvirus protein kinase.
| Project/Area Number |
22890039
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Virology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KATO Akihisa 東京大学, 医科学研究所, 助教 (40581187)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 単純ヘルペスウイルス / リン酸化 / 病態発現 |
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| Research Abstract |
Herpes simplex virus-1 (HSV-1) encodes at least two protein kinases, Us3 and UL13. In this study, Us3 in vitro kinase assay system shown that Us3 phosphorylates viral protein UL47 at Ser-77 and dUTPase at Ser-187. Interestingly, replacement of UL47 Ser-77 by alanine reduced viral replication in the mouse cornea and the development of herpes stromal keratitis in mice. In contrast, replacement of dUTPase Ser-187 by alanine reduced the HSV-1 neurovirulence.
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