2011 Fiscal Year Final Research Report
How epigenetic regulate lip formation
| Project/Area Number |
22890058
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthodontic/Pediatric dentistry
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Keywords | 矯正 / 口唇裂・口蓋裂 / エピジェネティック / ヒストン / メチル化 / 環境因子 |
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| Research Abstract |
The purpose of this study is to identify an involvement of epigenetic changes during cleft lip formation by environmental factors such as retinoic acid. We have accomplished making cleft lip by implanting bead that contained retinoic acid into the nasal pit of E3. 5 chick embryo. Using the same model, we have collected RNA from frontonasal mass and maxillary prominence to see the changes how retinoic acid affect histone metyltransferases or demethyltransferase that covalently modify lysine 9 of histone H3. SETDB1 that tri-methylate histone 3 of lysine 9 was significantly downregulated by treatment of retinoic acid.
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