2011 Fiscal Year Final Research Report

Functional analysis of cancer-specific human T cells in NOG mice : Cell interaction of CD4, CD8 cells

Research Project

Project/Area Number	22890084
Research Category	Grant-in-Aid for Research Activity Start-up
Allocation Type	Single-year Grants
Research Field	General surgery
Research Institution	Mie University
Principal Investigator	WANG Linan 三重大学, 大学院・医学系研究科, 特任助教 (00589484)
Project Period (FY)	2010 – 2011
Keywords	MAGE-A4 / NOG mouse / CEA / T-body
Research Abstract	Adoptive cell therapy(ACT) for cancer, in which T cells are collected from patient's peripheral blood, modified by the transduction of tumor-antigen specific T cell receptor(TCR) and re-administered to the same patient, is increasingly the subject of clinical trials. In this study, we aimed to establish a mouse model to analyze "in vivo" functions of the transferred T cells by utilizing immunocompromised(NOG) mice. We observed the regression of transplanted human esophageal tumor(HLA-A2402+, MAGE-A4+) growth only when T cells modified to express TCR, recognizing MAGE-A4(p143-151) peptide in the context of HLA-A2402, were co-administered. This model will provide us more detailed information about mechanism of efficacies of transferred T cells.