2011 Fiscal Year Final Research Report
Real time kinetic analysis of the skin dendritic cells in the steady state and various stimulus conditions.
| Project/Area Number |
22890093
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Dermatology
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Keywords | 皮膚樹状細胞 / ランゲルハンス細胞 / mDia |
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| Research Abstract |
The factors that determine the diversity of the immune response in the skin is still largely unknown. The purpose of this study was to elucidate its role and identification of molecules involved in the immune responses, especially against external stimuli.
We observed the dynamics behavior of dendritic cells of the skin using a Langerin-eGFP mouse over a period of time in the steady state, a relatively weak stimulus such as tape strip, and applied fluorescence-labeled hapten and established the real-time imaging method in vivo.
In addition, we have made it possible to evaluate the dynamics and cell migration of immune cells in the skin after that we transfer intravenously differentiated and cultured T cellsinto type Th1and Th2 T cells(Egawa et. Al. JID 2011 Apr).
And more, we have shown that mDia1, which is one of the cytoskeletal molecules in the Rho family, play an essential role in the phagocytosis, migration, adhesion of skin dendritic cells, and immune synapse formation with the another immune cells, such as immunological synapse.
Finally, we repoted that the contact dermatitis response is not formed at the time of the failure of this system(tanizaki et. al. Blood 2010 Dec)
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