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2011 Fiscal Year Final Research Report

Development of a new rapid bacterial identification using bacteriophage tail ligand molecule

Research Project

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Project/Area Number 22890129
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Infectious disease medicine
Research InstitutionKochi University

Principal Investigator

UCHIYAMA Jumpei  高知大学, 教育研究部・医療学系, 助教 (20574619)

Research Collaborator DAIBATA Masanori  高知大学, 教育研究部・医療学系, 教授
MATSUZAKI Shigenobu  高知大学, 教育研究部・医療学系, 准教授
UCHIYAMA Iyo  高知大学, 医学部附属病院・検査部, 技師
Project Period (FY) 2010 – 2011
Keywordsバクテリオファージ / ファージ / 細菌検出法 / ファージリガンド
Research Abstract

Infection control is extremely important to prevent infection spread. Rapid bacterial examination in infection control enables early treatment of patients, reduction of medical costs and prevention of infection spread. Thus, the development of more rapid bacterial detection systems is urgently required. The principal Investigator focused on the specific bacteriophage(phage ; bacterial virus) structural proteins contributed to the bacteria-species-specific binding ability, and examined the applicability of the phage molecule to bacterial detection system. As a result, the phage tail molecule was revealed to be potentially used for the rapid bacteria detection system.

  • Research Products

    (29 results)

All 2012 2011 2010

All Journal Article (8 results) (of which Peer Reviewed: 7 results) Presentation (19 results) Book (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Analysis of deoxynucleosides in bacteriophagesφEF24C and K and the frequency of a specific restriction site in the genomes of bacteriophage subfamily Spounavirinae2012

    • Author(s)
      Uchiyama J, Maeda Y, Takemura I, Gamoh K, Matsuzaki S, Daibata M
    • Journal Title

      Archive of Virology

    • Peer Reviewed
  • [Journal Article] Genetic characterization of Pseudomonas aeruginosa bacteriophage KPP102012

    • Author(s)
      Uchiyama J, Rashel M, Takemura I, Kato S, Ujihara T, Muraoka A, Matsuzaki S, Daibata M
    • Journal Title

      Archive of Virology

      Volume: 157(4) Pages: 733-738

    • Peer Reviewed
  • [Journal Article] Improved adsorption of an Enterococcus faecalis bacteriophageφEF24C with a spontaneous point mutation2011

    • Author(s)
      Uchiyama J, Takemura I, Satoh M, Kato S, Ujihara T, Akechi K, Matsuzaki S, Daibata M
    • Journal Title

      PLoS ONE

      Volume: 6(10) Pages: e26648

    • URL

      http://dx.plos.org/10.1371/journal.pone.0026648

    • Peer Reviewed
  • [Journal Article] Urothelial/ lamina propria spontaneous activity and the role of M3 muscarinic receptors in mediating rate responses to stretch and carbachol2011

    • Author(s)
      Moro C, Uchiyama J, Chess-Williams R
    • Journal Title

      Urology

      Volume: 78(6): 1442 Pages: e9-15

    • Peer Reviewed
  • [Journal Article] バクテリオファージ療法研究の現状と展望2011

    • Author(s)
      松崎茂展、内山淳平、竹村伊代、大畑雅典
    • Journal Title

      日本医事新報

      Volume: 4551 Pages: 48-49

  • [Journal Article] Characterization of lytic enzyme open reading frame 9(ORF9) derived from Enterococcus faecalis bacteriophageφEF24C2011

    • Author(s)
      Uchiyama J, Takemura I, Hayashi I, Matsuzaki S, Satoh M, Ujihara T, Murakami M, Imajoh M, Sugai M, Daibata M
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 77(2) Pages: 580-585

    • Peer Reviewed
  • [Journal Article] Presence of Merkel cell polyomavirus in Japanese cutaneous squamous cell carcinoma2011

    • Author(s)
      Murakami M, Imajoh M, Ikawa T, Nakajima H, Kamioka M, Nemoto Y, Ujihara T, Uchiyama J, Matsuzaki S, Sano S, Daibata M
    • Journal Title

      Journal of Clinical Virology

      Volume: 50(1) Pages: 37-41

    • Peer Reviewed
  • [Journal Article] Isolation and characterization of a novel Staphylococcus aureus bacteriophage,φMR25, and its therapeutic potential2010

    • Author(s)
      Hoshiba H, Uchiyama J, Kato S, Ujihara T, Muraoka A, Daibata M, Wakiguchi H, Matsuzaki S
    • Journal Title

      Archives of virology

      Volume: 155(4) Pages: 545-52

    • Peer Reviewed
  • [Presentation] 緑膿菌臨床分離株に対するバクテリオファージKPP12の溶菌効果の検討.2012

    • Author(s)
      福田憲,内山淳平,森田珠恵,石田わか,角環,松崎茂展,大畑雅典,福島敦樹
    • Organizer
      角膜カンファランス2012
    • Place of Presentation
      ホテルニューオータニ東京都千代田区
    • Year and Date
      20120000
  • [Presentation] Improved adsorption of an Enterococcus faecalis bacteriophageφEF24C with a point mutation2012

    • Author(s)
      Jumpei Uchiyama, Iyo Takemura, Takako Ujihara, Shigenobu Matsuzaki, Masanori Daibata
    • Organizer
      第85回日本細菌学会総会
    • Place of Presentation
      長崎
    • Year and Date
      2012-03-27
  • [Presentation] バクテリオファージの尾部リガンド分子を利用する細菌同定法2012

    • Author(s)
      松崎茂展、内山淳平、内山(竹村)伊代、大畑雅典
    • Organizer
      国際医薬品原料・中間体展2012(CPhIJAPAN2012)
    • Place of Presentation
      東京
    • Year and Date
      2012-03-21
  • [Presentation] 黄色ブドウ球菌ファージの尾部リガンドタンパク質を利用した細菌検出法の開発2012

    • Author(s)
      内山伊代,内山淳平,松崎茂展,杉浦哲朗,大畑雅典
    • Organizer
      第8回合同地方会(第57回日本臨床検査医学会中国・四国支部総会,第152回日本臨床化学会中国支部例会・総会,第22回日本臨床化学会四国支部例会・総会)
    • Place of Presentation
      岡山
    • Year and Date
      2012-02-05
  • [Presentation] 緑膿菌ファージKPP10のゲノムDNA解析2011

    • Author(s)
      内山淳平,竹村伊代,氏原隆子,邑岡麻子,松崎茂展,大畑雅典
    • Organizer
      第64回日本細菌学会中国・四国支部総
    • Place of Presentation
      岡山
    • Year and Date
      2011-10-22
  • [Presentation] 黄色ブドウ球菌ファージS13'およびS24-1ゲノム比較による尾部吸着タンパク質の同定と解析2011

    • Author(s)
      内山淳平,竹村伊代,佐藤美帆,加藤伸一郎,氏原隆子,松崎茂展,大畑雅典
    • Organizer
      第56回日本ブドウ球菌研究会
    • Place of Presentation
      高知
    • Year and Date
      2011-09-24
  • [Presentation] Improved adsorption of Enterococcus faecalis bacteriophageφEF24C caused by a point mutation in a tail fiber gene2011

    • Author(s)
      Uchiyama J, Takemura I, Satoh M, Kato S, Ujihara T, Akechi K, Matsuzaki S, Daibata M
    • Organizer
      Symposium, International Congress of Virology
    • Place of Presentation
      札幌
    • Year and Date
      2011-09-16
  • [Presentation] Identification of a tail adsorption protein by comparative genomic analysis of Staphylococcus aureus bacteriophages S13' and S24-1. Symposium2011

    • Author(s)
      Takemura I, Uchiyama J, Satoh M, Kato S, Ujihara T, Akechi K, Matsuzaki S, Daibata M
    • Organizer
      International Congress of Virology
    • Place of Presentation
      札幌
    • Year and Date
      2011-09-16
  • [Presentation] 感染効率が改善された腸球菌バクテリオファージφEF24C変異体の解析2011

    • Author(s)
      内山淳平,竹村伊代,佐藤美帆,加藤伸一郎,氏原隆子,明智和愛,松崎茂展,大畑雅典
    • Organizer
      第5回細菌学・若手コロッセウム
    • Place of Presentation
      高知
    • Year and Date
      2011-08-09
  • [Presentation] バクテリオファージによるマウス緑膿菌角膜炎の抑制効果2011

    • Author(s)
      福田憲,石田わか,角環,森田珠恵,内山淳平,松崎茂展,大畑雅典,福島敦樹
    • Organizer
      第48回日本眼感染症学会,国立京都国際会館
    • Place of Presentation
      京都市
    • Year and Date
      2011-07-08
  • [Presentation] 黄色ブドウ球菌バクテリオファージ(ファージ) S13'及びS24-1の比較ゲノム解析による尾部吸着タンパク質の同定.学部長裁量経費採択演題2011

    • Author(s)
      内山淳平,竹村伊代,氏原隆子,佐藤美帆,松崎茂展,今城雅之,村上雅尚,大畑雅典
    • Organizer
      第10回Kochi Medical School(KMS) Research Meeting
    • Place of Presentation
      高知
    • Year and Date
      2011-02-02
  • [Presentation] 巨大黄色ブドウ球菌バクテリオファージの解析2010

    • Author(s)
      内山淳平,竹村伊代,氏原隆子,松崎茂展,村上雅尚,大畑雅典
    • Organizer
      第63回日本細菌学会中国・四国支部総会
    • Place of Presentation
      愛媛・松山
    • Year and Date
      2010-10-16
  • [Presentation] 黄色ブドウ球菌バクテリオファージ(ファージ) S13'及びS24-1の比較ゲノム解析による尾部吸着タンパク質の同定2010

    • Author(s)
      内山淳平,竹村伊代,氏原隆子,松崎茂展,村上雅尚,大畑雅典
    • Organizer
      第63回日本細菌学会中国・四国支部総会
    • Place of Presentation
      愛媛・松山
    • Year and Date
      2010-10-16
  • [Presentation] ファージ療法およびファージを利用する新しい細菌検出法の開発:高知大学の取り組み,大阪大学蛋白質研究所セミナー2010

    • Author(s)
      内山淳平,松崎茂展,大畑雅典
    • Organizer
      バクテリオファージ研究の可能性と課題
    • Place of Presentation
      大阪
    • Year and Date
      2010-09-09
  • [Presentation] 黄色ブドウ球菌バクテリオファージS13'及びS24-1の比較ゲノム解析と尾部吸着タンパク質の特徴付け,大阪大学蛋白質研究所セミナー2010

    • Author(s)
      竹村伊代,内山淳平,氏原隆子,松崎茂展,村上雅尚,今城雅之,大畑雅典
    • Organizer
      バクテリオファージ研究の可能性と課題
    • Place of Presentation
      大阪
    • Year and Date
      2010-09-09
  • [Presentation] 新規黄色ブドウ球菌バクテリオファージS6の解析,大阪大学蛋白質研究所セミナー2010

    • Author(s)
      内山淳平,竹村伊代,氏原隆子,松崎茂展,村上雅尚,今城雅之,大畑雅典
    • Organizer
      バクテリオファージ研究の可能性と課題
    • Place of Presentation
      大阪
    • Year and Date
      2010-09-09
  • [Presentation] 腸球菌ファージφEF24C由来溶菌酵素ORF9の解析2010

    • Author(s)
      内山淳平,林幾江,竹村伊代,氏原隆子,竹内啓晃,杉浦哲朗,菅井基之,大畑雅典,松崎茂展
    • Organizer
      第83回日本細菌学会総会
    • Place of Presentation
      横浜
    • Year and Date
      2010-03-29
  • [Presentation] 新規Serratia marcescensバクテリオファージKSP20, KSP90,およびKSP100の性状解析2010

    • Author(s)
      松下憲司,内山淳平,氏原隆子,杉原重喜,邑岡麻子,大畑雅典,松崎茂展
    • Organizer
      第83回日本細菌学会総会
    • Place of Presentation
      横浜
    • Year and Date
      2010-03-29
  • [Presentation] 腸球菌ファージφEF24C由来溶菌酵素ORF9の解析2010

    • Author(s)
      内山淳平、竹村伊代、氏原隆子、根本由以子、今城雅之、村上政尚、大畑雅典、松崎茂展
    • Organizer
      第10回Kochi Medical School(KMS) Research Meeting
    • Place of Presentation
      高知
    • Year and Date
      2010-02-03
  • [Book] Caister Academic Press2012

    • Author(s)
      Matsuzaki S, Uchiyama J, Daibata M
    • Publisher
      Phage therapy : experiments using animal infection models
  • [Patent(Industrial Property Rights)] 黄色ブドウ球菌に結合するタンパク質及びそのタンパク質を利用した黄色ブドウ球菌の測定方法2011

    • Inventor(s)
      内山淳平、竹村伊代、松崎茂展、大畑雅典
    • Industrial Property Rights Holder
      国立大学法人高知大学
    • Industrial Property Number
      特許、特願2011-192996
    • Filing Date
      2011-09-05

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Published: 2013-07-31  

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