2011 Fiscal Year Final Research Report
Analysis of ubiquitination pathway to intracytoplasmic bacteria
Project/Area Number |
22890154
|
Research Category |
Grant-in-Aid for Research Activity Start-up
|
Allocation Type | Single-year Grants |
Research Field |
Bacteriology (including Mycology)
|
Research Institution | University of Shizuoka |
Principal Investigator |
YOSHIKAWA Yuko 静岡県立大学, 食品栄養科学部, 助教 (00580523)
|
Project Period (FY) |
2010 – 2011
|
Keywords | 細菌 / 感染症 / リステリア / オートファジー / ユビキチン |
Research Abstract |
Ubiquitinated ActA-deficient Listeria monocytogenes(. actA) were recognized by autophagy. To elucidate the mechanism of Listeria ubiquitination, we attempted to explore the bacterial ubiquitinized molecules and the host ubiquitination factors. As a result, two candidates of bacterial ubiquitinated molecules and four candidates involved in host ubiquitination system were identified by LC-MS/MS analysis. The recombinant proteins of Listeria candidate moleculesexamined by in vitro ubiquitination assay, and one of the two candidates inListeria wa weres received ubiquitination directly.
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