2011 Fiscal Year Final Research Report
The determination of the hSNF5 target genes and the clarification of the transcriptional mechanism of hSNF5
| Project/Area Number |
22890157
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Pediatrics
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Keywords | ラブドイド腫瘍 / hSNF5 / NOXA |
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| Research Abstract |
Malignant rhabdoid tumor(MRT), a highly aggressive cancer of young children, displays inactivation of the hSNF5 gene in primary tumors and cell lines. We have previously reported that reexpression of hSNF5 in MRT cell lines causes a G1 arrest via p21WAF1/CIP1(p21) mRNA induction. However, the mechanisms of p21 promoter activation by hSNF5 remain unclear.
Because p21 is a transcriptional target of the p53 tumor suppressor gene, we determined the role of p53 in hSNF5-induced p21 activation. We reexpressed hSNF5 in the A204 and TTC642 MRT cell lines, with or without stable knockdown of p53 expression, using adenoviral vectors expressing either hSNF5 and GFP or GFP. While loss of p53 expression significantly inhibited p21 transcriptional activity induced by hSNF5 in A204 cells at 24 hours, it did not alter its activity by hSNF5 after 24 hours in the TTC642 cells. These results suggested that the up-regulation p21 transcription by hSNF5 operated through p53-dependent and. independent mechani
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sms in the A204 and TTC642 cell lines, respectively. Next, we used chromatin immunoprecipitation(ChIP) analysis of the p21 promoter to determine where hSNF5 appeared at the p21 promoter and whether its recruitment altered binding of other transcription factors or the chromatin landscape. Our results indicated that reexpressed hSNF5 binds within 1 kb of transcript start site(TSS), with maximal enrichment at the TSS in both cell lines. Furthermore, histone acetyltransferases(HATs), RNA polymerase II(RNAPII), and BRG-1 are assembled with p53 and CDK8(co-activator of p53 transcriptional program) by reexpression of hSNF5 in A204 cells. In contrast, while p53 and CDK8 occupancy did not change after hSNF5 reexpression in the TTC642 cells, HATs, RNAPII, and BRG-1 levels increased at the p21 promoter. These findings correlated with the results of p53 knock-down experiments. Furthermore, histone H3K36 tri-methylation increased downstream of the TSS in both cell lines. These results suggested hSNF5 regulates the initiation activity of the p21 promoter by either itself or p53 recruitment, resulting in the mRNA elongation.
Our findings demonstrate that while induction of p21 transcription by hSNF5 in A204 cells depends on p53, it occurs independently of p53 in TTC642 cells. The lack of dependence upon p53 appears to extend to other MRT cell lines tested in our laboratory. Furthermore, our results suggest that hSNF5 may alter p21 transcriptional initiation by itself or through the recruitment of other uncharacterized transcription factors. Less
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[Journal Article] Sensitivity of malignant rhabdoid tumor cell lines to PD0332991 is inverserly correlated with p16 expression2011
Author(s)
Katsumi Y, Iehara T, Miyachi M, Yagyu Y, Tsubai-Shimizu T, Kikuchi K, Tamura S, Itoh H, Kuwahara Y, Tsuchiya K, Kuroda H, Sugimoto T, Houghton PJ, Hosoi H
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Journal Title
Biochem Biophys Res Commun
Volume: 413
Pages: 62-68
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