2011 Fiscal Year Final Research Report
Creation of a novel therapeutic antibody for tumor metastasis-Targeting Tumor Vasculogenic Mimicry-
| Project/Area Number |
22890192
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Physical pharmacy
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| Research Institution | Teikyo University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Keywords | 転移 / 腫瘍擬似血管 / ファージ表面提示法 / 免疫抗体ライブラリ |
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| Research Abstract |
In this study, we had an attempt to develop a novel therapy for tumor. Recent years, it is reported that in the tumor tissue tumor cells compose luminal structure as like vessel. This structure is called as tumor vasculogenic mimicry(TVM). There are many red blood cells in a part of TVM. It is expected that TVM has an important role to metastasize via blood flow. Therefore, it expects that the inhibition of both angiogenesis and TVM can result in the inhibition of tumor growth and metastasis. Therefore, we had an attempt to create anti-TVM antibody for tumor therapy, using phage-display technique. Next, we evaluated tumor growth suppressive effect by TVM vaccine therapy for tumor bearing mice to clarify how TVM development associates with metastasis. As the result, in TVM-immune mice, tumor growth had a tendency to be suppressed, compared with non-treated mice. In addition, to evaluate TVM property as blood vessel, we measured enzyme activity which has high activity in endothelial cell. As the result, in TVM, we can't confirm the elevated activity. Thus, we clarified that TVM possess properties differing from blood vessel in spite of being similar in structure to them.
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[Journal Article] Fine tuning of receptor-selectivity for tumor necrosis factor-α using a phage display system with one-step competitive panning2011
Author(s)
Abe Y, Yoshikawa T, Inoue M, Nomura T, Furuya T, Yamashita T, Nagano K, Nabeshi H, Yoshioka Y, Mukai Y, Nakagawa S, Kamada H, Tsutsumi Y, Tsunoda S
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Journal Title
Biomaterials
Volume: 32(23)
Pages: 5498-5504
Peer Reviewed
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[Journal Article] Therapeutic effect of PEGylated TNFR1-selective antagonistic mutant TNF in experimental autoimmune encephalomyelitis mice2011
Author(s)
Nomura T, Abe Y, Kamada H, Shibata H, Kayamuro H, Inoue M, Kawara T, Arita S, Furuya T, Yamashita T, Nagano K, Yoshikawa T, Yoshioka Y, Mukai Y, Nakagawa S, Taniai M, Ohta T, Serada S, Naka T, Tsunoda SI, Tsutsumi Y
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Journal Title
J. Control. Release
Volume: 149(1)
Pages: 8-14
Peer Reviewed
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[Journal Article] Arsenic trioxide induces down-regulation of gp46 via protein oxidation : proteomics analysis of oxidative modified proteins in As2O3-treated HTLV-1-infected cells2010
Author(s)
Nabeshi H, Yoshikawa T, Kamada H, Shibata H, Sugita T, Abe Y, Nagano K, Nomura T, Minowa K, Tsunoda S, Tsutsumi Y
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Journal Title
Pharmazie
Volume: 65(9)
Pages: 702-707
Peer Reviewed
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[Journal Article] Interleukin-1 family cytokines as mucosal vaccine adjuvants for induction of protective immunity against influenza virus2010
Author(s)
Kayamuro H, Yoshioka Y, Abe Y, Arita S, Katayama K, Nomura T, Yoshikawa T, Kubota-Koketsu R, Ikuta K, Okamoto S, Mori Y, Kunisawa J, Kiyono H, Itoh N, Nagano K, Kamada H, Tsutsumi Y, Tsunoda S
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Journal Title
J Virol
Volume: 84(24)
Pages: 12703-12
Peer Reviewed
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