2011 Fiscal Year Final Research Report
The assembly of an animal experimental model system for cell transplantation therapy using allogeneic stem cells with severe cardiac failure.
| Project/Area Number |
22890249
|
|---|
| Research Category |
Grant-in-Aid for Research Activity Start-up
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
General surgery
|
|---|
| Research Institution | Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology |
|---|
Principal Investigator |
ITAKURA Yoko 地方独立行政法人東京都健康長寿医療センター(東京都健康長寿医療センター研究所), 東京都健康長寿医療センター研究所, 研究員 (30582746)
|
|---|
| Project Period (FY) |
2010 – 2011
|
| Keywords | 再生医学 / 心臓 / 大動物 / 評価 / 幹細胞移植 / 間葉系細胞 |
|---|
| Research Abstract |
We established porcine amniotic membrane, placenta and umbilical cord-derived mesenchymal cells. Their mesenchymal cells were expressed mesencymal stem cell markers, just as human cells. The cells transduced with enhanced green fluorescent protein(EGFP) were directly transplanted in injured myocardium on porcine chronic myocardial ischemic model. Injected allogeneic EGFP-expressing AMSCs were identified in the immunocompetent host heart without the use of any immunosuppressants at 4 weeks after transplantation. Immunohistochemistry revealed that EGFP colocalized with cardiac troponin T and cardiac troponin I.
|
